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Type: Artigo de periódico
Title: Evaluation of the cytotoxic and genotoxic potential of water from the River Paraiba do Sul, in Brazil, with the Allium cepa L. test
Author: Barberio, A
Barros, L
Voltolini, JC
Mello, MLS
Abstract: This work investigated the cytotoxic and genotoxic potential of water from the River Paraiba do Sul (Brazil) using Allium cepa roots. An anatomo-morphological parameter (root length), mitotic indices, and frequency of micronuclei were analysed. Eight bulbs were chosen at random for treatment for 24 to 120 hours with the River water collected in the years of 2005 and 2006 from sites in the cities of Tremembe and Aparecida (Sao Paulo state, Brazil). Daily measurements of the length of the roots grown from each bulb were carried out throughout the experiment. Mitotic index (MI) and frequency of micronuclei (MN) were determined for 2000 cells per root, using 3-5 root tips from other bulbs (7-10). Only in the roots treated with samples of the River water collected in 2005 in Tremembe city was there a decrease in the root length growth compared to the respective control. However, a reduction in MI values was verified for both sites analysed for that year. Considering the data involving root length growth and especially MI values, a cytotoxic potential is suggested for the water of the River Paraiba do Sul at Tremembe and Aparecida, in the year of 2005. On the other hand, since in this year the MN frequency was not affected with the river water treatments. genotoxicity is not assumed for the river water sampled at the aforementioned places.
Subject: cytotoxicity
Allium cepa
mitotic index
River Paraiba do Sul
Country: Brasil
Editor: Int Inst Ecology
Rights: aberto
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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