Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/65902
Full metadata record
DC FieldValueLanguage
dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleEvaluation of sample preparation protocols for proteomic analysis of sunflower leavespt_BR
dc.contributor.authorda Silva, MAOpt_BR
dc.contributor.authorGarcia, JSpt_BR
dc.contributor.authorde Souza, GHMFpt_BR
dc.contributor.authorEberlin, MNpt_BR
dc.contributor.authorGozzo, FCpt_BR
dc.contributor.authorArruda, MAZpt_BR
unicamp.author.emailzezzi@iqm.unicamp.brpt_BR
unicamp.authorOseas da Silva, Marcelo Anselmo Zezzi Arruda, Marco Aurelio Univ Estadual Campinas, Inst Chem, Grp Spectrometry Sample Preparat & Mech GEPAM, BR-13084862 Campinas, SP, Brazilpt_BR
unicamp.authorOseas da Silva, Marcelo Anselmo Zezzi Arruda, Marco Aurelio Univ Estadual Campinas, Inst Chem, Natl Inst Sci & Technol Bioanalyt, BR-13083970 Campinas, SP, Brazilpt_BR
unicamp.authorGarcia, Jerusa Simone Martins Ferreira de Souza, Gustavo Henrique Eberlin, Marcos Nogueira Univ Estadual Campinas, Inst Chem, ThoMSon Mass Spectrometry Lab, BR-13084862 Campinas, SP, Brazilpt_BR
unicamp.authorMartins Ferreira de Souza, Gustavo Henrique Univ Estadual Campinas, Fac Med Sci, Dept Pharmacol, BR-13083887 Campinas, SP, Brazilpt_BR
unicamp.authorGozzo, Fabio Cesar Univ Estadual Campinas, Inst Chem, Dept Organ Chem, BR-13084862 Campinas, SP, Brazilpt_BR
dc.subject2D electrophoresispt_BR
dc.subjectSunflowerpt_BR
dc.subjectSample preparationpt_BR
dc.subjectMALDI-Q-TOF MSpt_BR
dc.subjectCross species identificationpt_BR
dc.subject.wos2-dimensional Gel-electrophoresispt_BR
dc.subject.wosMass-spectrometrypt_BR
dc.subject.wosPlant-tissuespt_BR
dc.subject.wosProtein Extractionpt_BR
dc.subject.wosOilpt_BR
dc.subject.wos2-dept_BR
dc.description.abstractSix protocols for extraction of proteins from sunflower (Helianthus annuus L.) leaves were evaluated for their abilities in both removing interferents and attaining the best resolution in two-dimensional gel electrophoresis. "Classical" phenol extraction followed by precipitation with ammonium acetate in methanol displayed the most efficient protocol, which allowed the detection of 244 protein spots with ca. 485 mu g of protein in gel electrophoresis. Tandem mass spectrometry was performed to identify proteins in 61 spots, and cross species identification was used for this task. Proteins from twenty two spots were identified, and 12 of these proteins are up to now not included into the ExPASy sunflower protein databank. (C) 2009 Elsevier B.V. All rights reserved.pt
dc.relation.ispartofTalantapt_BR
dc.relation.ispartofabbreviationTalantapt_BR
dc.publisher.cityAmsterdampt_BR
dc.publisher.countryHolandapt_BR
dc.publisherElsevier Science Bvpt_BR
dc.date.issued2010pt_BR
dc.date.monthofcirculationFEB 15pt_BR
dc.identifier.citationTalanta. Elsevier Science Bv, v. 80, n. 4, n. 1545, n. 1551, 2010.pt_BR
dc.language.isoenpt_BR
dc.description.volume80pt_BR
dc.description.issuenumber4pt_BR
dc.description.issuespecialSIpt_BR
dc.description.firstpage1545pt_BR
dc.description.lastpage1551pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policypt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0039-9140pt_BR
dc.identifier.wosidWOS:000273921900010pt_BR
dc.identifier.doi10.1016/j.talanta.2009.06.016pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipFinanciadora de Estudos e Projetos (FINEP, Brasilia, Brazil)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.date.available2014-11-17T13:12:46Z
dc.date.available2015-11-26T17:38:10Z-
dc.date.accessioned2014-11-17T13:12:46Z
dc.date.accessioned2015-11-26T17:38:10Z-
dc.description.provenanceMade available in DSpace on 2014-11-17T13:12:46Z (GMT). No. of bitstreams: 1 WOS000273921900010.pdf: 462955 bytes, checksum: d52eefb9135a5842d8f2d36828604c46 (MD5) Previous issue date: 2010en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:38:10Z (GMT). No. of bitstreams: 2 WOS000273921900010.pdf: 462955 bytes, checksum: d52eefb9135a5842d8f2d36828604c46 (MD5) WOS000273921900010.pdf.txt: 32815 bytes, checksum: 437fba6f810c636a5a773cfa88a7e926 (MD5) Previous issue date: 2010en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/65902pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/65902
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/65902-
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
WOS000273921900010.pdf452.1 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.