Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/65902
Type: Artigo de periódico
Title: Evaluation of sample preparation protocols for proteomic analysis of sunflower leaves
Author: da Silva, MAO
Garcia, JS
de Souza, GHMF
Eberlin, MN
Gozzo, FC
Arruda, MAZ
Abstract: Six protocols for extraction of proteins from sunflower (Helianthus annuus L.) leaves were evaluated for their abilities in both removing interferents and attaining the best resolution in two-dimensional gel electrophoresis. "Classical" phenol extraction followed by precipitation with ammonium acetate in methanol displayed the most efficient protocol, which allowed the detection of 244 protein spots with ca. 485 mu g of protein in gel electrophoresis. Tandem mass spectrometry was performed to identify proteins in 61 spots, and cross species identification was used for this task. Proteins from twenty two spots were identified, and 12 of these proteins are up to now not included into the ExPASy sunflower protein databank. (C) 2009 Elsevier B.V. All rights reserved.
Subject: 2D electrophoresis
Sunflower
Sample preparation
MALDI-Q-TOF MS
Cross species identification
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Identifier DOI: 10.1016/j.talanta.2009.06.016
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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