Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/659
Type: Artigo de periódico
Title: Functional association of human Ki-1/57 with pre-mRNA splicing events
Author: BRESSAN, Gustavo C.
QUARESMA, Alexandre J. C.
MORAES, Eduardo C.
MANFIOLLI, Adriana O.
PASSOS, Dario O.
GOMES, Marcelo D.
KOBARG, Joerg
Abstract: The cytoplasmic and nuclear protein Ki- 1 / 57 was first identified in malignant cells from Hodgkin`s lymphoma. Despite studies showing its phosphorylation, arginine methylation, and interaction with several regulatory proteins, the functional role of Ki- 1 / 57 in human cells remains to be determined. Here, we investigated the relationship of Ki- 1 / 57 with RNA functions. Through immunoprecipitation assays, we verified the association of Ki- 1 / 57 with the endogenous splicing proteins hnRNPQ and SFRS9 in HeLa cell extracts. We also found that recombinant Ki- 1 / 57 was able to bind to a poly- U RNA probe in electrophoretic mobility shift assays. In a classic splicing test, we showed that Ki- 1 / 57 can modify the splicing site selection of the adenoviral E1A minigene in a dose- dependent manner. Further confocal and. uorescence microscopy analysis revealed the localization of enhanced green. uorescent protein - Ki- 1 / 57 to nuclear bodies involved in RNA processing and or small nuclear ribonucleoprotein assembly, depending on the cellular methylation status and its N- terminal region. In summary, our findings suggest that Ki- 1 / 57 is probably involved in cellular events related to RNA functions, such as pre- mRNA splicing.
Subject: Arg methylation
nuclear bodies
protein-protein interaction
RNA binding
splicing
Country: Estados Unidos
Editor: WILEY-BLACKWELL PUBLISHING, INC
Citation: FEBS JOURNAL, v.276, n.14, p.4194-4207, 2009
Rights: fechado
Identifier DOI: 10.1111/j.1742-4658.2009.07092.x
Address: http://apps.isiknowledge.com/InboundService.do?Func=Frame&product=WOS&action=retrieve&SrcApp=EndNote&UT=000267282400007&Init=Yes&SrcAuth=ResearchSoft&mode=FullRecord
http://dx.doi.org/10.1111/j.1742-4658.2009.07092.x
Date Issue: 2009
Appears in Collections:IB - Artigos e Materiais de Revistas Científicas

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