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|Type:||Artigo de periódico|
|Title:||Evaluation of Nitric Oxide (NO) and Nitric Oxide Synthases (NOS) in the Amniotic Fluid in an Experimental Gastroschisis Rat Model|
de Souza, GFP
de Oliveira, MG
|Abstract:||Intestinal damage due to gastroschisis (G), an anomaly found with increasing incidence by pedriatic surgeons, is intimately associated with endogenous nitric oxide (NO) production and NO synthase (NOS) expression. Aim: Aim of the study was to evaluate NO production and NOS isoforms in the intestine and amniotic fluid (AF) using a rat model of gastroschisis. Methods: A gastroschisis rat model was surgically created at 18.5 days of gestation (term = 22 days). 3 groups of 12 fetuses each were studied: control (C), sham (S) and (G). Morphometric data of body weight (BW), intestinal weight (IW) and the IW/BW ratio were evaluated and compared. Indirect quantification of NO (nitrite and nitrate - NOx) was analyzed by chemiluminescence, and the expression of the 3 isoforms was analyzed by Western blotting. Results: Group G showed an increase in IW and IW/BW compared with groups C and S. IW: G = 0.27 +/- 0.06, C = 0.20 +/- 0.02, S = 0.20 +/- 0.02 (p < 0.01); IW/BW: G = 4.11 +/- 0.57, C = 5.21 +/- 1.04, S = 5.18 +/- 1.23 (p < 0.05). NO in the G group was lower in the intestine and higher in AF, as opposed to C and S, where it had increased in the intestine and decreased in AF. Intestinal NOx: G = 0.85 +/- 0.28, C = 1.86 +/- 0.82, S = 1.80 +/- 0.69 (p < 0.05); NOx in AF: G = 161.87 +/- 52.11, C = 6.99 +/- 5.45, S = 48.73 +/- 13.183 (p < 0.001). Conclusion: The intestinal inflammation in gastroschisis promotes the release of nitric oxide to the environment (AF). Perhaps NO in the AF may be an inflammatory marker for G.|
nitric oxide synthases
|Editor:||Georg Thieme Verlag Kg|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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