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Type: Artigo de periódico
Title: Evaluation of immobilized metal membrane affinity chromatography for purification of an immunoglobulin G(1) monoclonal antibody
Author: Serpa, G
Augusto, EFP
Tamashiro, WMSC
Ribeiro, MB
Miranda, EA
Bueno, SMA
Abstract: The large scale production of monoclonal antibodies (McAbs) has gaining increased relevance with the development of the hybridoma cell culture in bioreactors creating a need for specific efficient bioseparation techniques. Conventional fixed bead affinity adsorption commonly applied for McAbs purification has the drawback of low flow rates and colmatage. We developed and evaluated a immobilized metal affinity chromatographies (IMAC) affinity membrane for the purification of anti-TNP IgG(1) mouse McAbs. We immobilized metal ions on a poly(ethylene vinyl alcohol) hollow fiber membrane (Me2+-IDA-PEVA) and applied it for the purification of this McAbs from cell culture supernatant after precipitation with 50% saturation of ammonium sulphate. The purity of IgG(1) in the eluate fractions was high when eluted from Zn2+ complex. The anti-TNP antibody could be eluted under conditions causing no loss of antigen binding capacity. The purification procedure can be considered as an alternative to the biospecific adsorbent commonly applied for mouse IgG(1) purification, the protein G-Sepharose. (C) 2004 Elsevier B.V. All rights reserved.
Subject: monoclonal antibody
affinity membrane
Country: Holanda
Editor: Elsevier Science Bv
Citation: Journal Of Chromatography B-analytical Technologies In The Biomedical And Life Sciences. Elsevier Science Bv, v. 816, n. 41671, n. 259, n. 268, 2005.
Rights: fechado
Identifier DOI: 10.1016/j.jchromb.2004.11.043
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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