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Type: Artigo de periódico
Title: Evaluation of Gelatinases, Tissue Inhibitor of Matrix Metalloproteinase-2, and Myeloperoxidase Protein in Healthy and Inflamed Human Dental Pulp Tissue
Author: Accorsi-Mendonca, T
Silva, EJNL
Marcaccini, AM
Gerlach, RF
Duarte, KMR
Pardo, APS
Line, SRP
Zaia, AA
Abstract: Introduction: The aim of this study was to compare the gelatinolytic activity of matrix metalloproteinase (MMP)-2 and MMP-9 and the expression of tissue inhibitor of matrix metalloproteinase (TIMP)-2 and myeloperoxidase protein (MPO) in clinically healthy human pulp and inflamed pulp tissue specimens. Methods: Twenty dental pulps clinically diagnosed as inflammatory tissues and 20 healthy pulp tissues from enclosed third molars were harvested and evaluated. The gelatinolytic activity for MMP-2 and MMP-9 was assessed by using the zymography technique, TIMP-2 gene expression was evaluated using the enzyme-linked immunosorbent assay, and MPO was determined using the MPO assay. Results: Data showed increased levels of MMP-9, active MMP-2, TIMP-2, and MPO in inflammatory pulp tissues compared with healthy tissues (P < .05). No statistical difference could be observed for pro-MMP-2 (P > .05). Conclusions: Although all samples were associated with MMP-2 expression, the active form of this M MP was observed only in inflamed pulps. Inflamed pulps showed an up-regulation of MMP-9, TIMP-2, and MPO.
Subject: Gelatinases
pulp biology
Country: EUA
Editor: Elsevier Science Inc
Citation: Journal Of Endodontics. Elsevier Science Inc, v. 39, n. 7, n. 879, n. 882, 2013.
Rights: fechado
Identifier DOI: 10.1016/j.joen.2012.11.011
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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