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Type: Artigo de periódico
Title: Evaluation of differential protein expression in Haliclona aquarius and sponge-associated microorganisms under cadmium stress
Author: Wanick, RC
Barbosa, HD
Frazao, LR
Santelli, RE
Arruda, MAZ
Coutinho, CC
Abstract: A comparative proteomic approach was used to assess differentially expressed proteins in marine sponges after 36 h of exposure to cadmium (Cd). After separation performed by 2-D polyacrylamide gel electrophoresis, 46 protein spots indicated differential expression, and 17 of these proteins were identified by electrospray ionization quadrupole time-of-flight mass spectrometry. From the proteins identified, 76 % were attributed to sponge-associated microorganisms (fungi and bacteria), and 24 % were attributed to Haliclona aquarius. Some of the proteins that were identified may be related to cell proliferation and differentiation or processes of oxidative stress repair and energy procurement. An integrated evaluation based on spot expression levels and the postulated functions of these proteins allowed a more accurate evaluation of the stress caused to the sponge holobiont system by cadmium exposure. This study could provide new insights into the use of a proteomic approach in the marine sponge to assess the effects of Cd pollution in a marine environment.
Subject: Sponge proteomics
2-D gel electrophoresis
Mass spectrometry
Stress response
Trace metals
Country: Alemanha
Editor: Springer Heidelberg
Citation: Analytical And Bioanalytical Chemistry. Springer Heidelberg, v. 405, n. 24, n. 7661, n. 7670, 2013.
Rights: fechado
Identifier DOI: 10.1007/s00216-013-6898-x
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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