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Type: Artigo de periódico
Title: Evaluation of Different Parameters for the Purification of Inulinase Using an Ion Exchange Fixed Bed
Author: Kalil, SJ
Silveira, ST
Maugeri, F
Rodrigues, MI
Abstract: The main conditions affecting the downstream process for purification of inulinase from Kluyveromyces marxianus var. bulgaricus ATCC 16045 were investigated. Parameters including the elution mode, linear rate, ionic strength, and elution pH were evaluated in order to achieve the best purification factor and recovery. The best purification factor and recovery were achieved in run 6 using a gradient elution mode with concentrations of NaCl ranging from 0 to 1 M, a linear flow rate of 100 cm/h and a pH value of 4.1. Under these conditions, the recovery was similar to 67.5% and the purification factor similar to 6.6-fold.
Subject: downstream process
fixed bed
ion exchange
Country: Coreia do Sul
Editor: Korean Soc Biotechnology & Bioengineering
Citation: Biotechnology And Bioprocess Engineering. Korean Soc Biotechnology & Bioengineering, v. 15, n. 4, n. 676, n. 679, 2010.
Rights: fechado
Identifier DOI: 10.1007/s12257-009-3018-z
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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