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Type: Artigo de periódico
Title: Evaluation of a novel kit (TF-test) for the diagnosis of intestinal parasitic infections
Author: Gomes, JF
Hoshino-Shimizu, S
Dias, LCS
Araujo, AJSA
Castilho, VLP
Neves, FAMA
Abstract: Intestinal parasitic infections are currently a source of concern for Public Health agencies in developing and developed countries. Since three ovum-and-parasite stool examinations have been demonstrated to provide sensitive results, we designed a practical and economical kit (TF-Test) that is now commercially available (Immunoassay Com. Ind. Ltda., S (a) over tildeo Paulo, Brazil). This kit allows the separate collection of three fecal specimens into a preservative solution. The specimens are then pooled, double-filtered, and concentrated by a single rapid centrifugation process. The TF-Test was evaluated in four different laboratories in a study using 1,102 outpatients and individuals living in an endemic area for enteroparasitosis. The overall sensitivity found using the TF-Test (86.2-97.8%) was significantly higher (P<0.01) than the sensitivity of conventional techniques such as the Coprotest (NIL Comercio Exterior Ltda, Sao Paulo, Brazil) and the combination of Lutz/Hoffman, Faust, and Rugai techniques (De Carli, Diagnostico Laboratorial das Parasitoses Humanas. Metodos e Tecnicas, 1994), which ranged from 48.3% to 75.9%. When the above combined three specimen technique was repeated with three specimens collected on different days, its sensitivity became similar (P > 0.01) to that of the TF-Test. The kappa index values of agreement for the TF-Test were consistent (P < 0.01), being higher and ranking in a better position than conventional techniques. The high sensitivity, cost/benefit ratio, and practical aspects demonstrate that the TF-Test is suitable for individual diagnosis, epidemiological inquiries, or evaluation of chemotherapy in treated communities. (C) 2004 Wiley-Liss, Inc.
Subject: TF-test
parasite-enrichment process
pooled three fecal specimen examinations
intestinal parasitic infections
Country: EUA
Editor: Wiley-liss
Citation: Journal Of Clinical Laboratory Analysis. Wiley-liss, v. 18, n. 2, n. 132, n. 138, 2004.
Rights: fechado
Identifier DOI: 10.1002/jcla.20011
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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