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Type: Artigo de periódico
Title: Establishment and partial characterization of a continuous human malignant glioma cell line: NG97
Author: Grippo, MC
Penteado, PF
Carelli, EF
Cruz-Hofling, MA
Verinaud, L
Abstract: 1. A human glioma cell line, NG97, was established from tissue obtained from a patient diagnosed with a grade III astrocytoma. 2. The NG97 cell line has been subcultured for more than, 100 passages in standard culture media without feeder layer or collagen coatings. 3. NG97 cells grow in vitro as two subpopulations with distinct morphological appearance: stellate cells with pleomorphic nuclei, and small round cells with few processes. The cells have a doubling time of about 72 It and a plating efficiency of 1%. The injection of NG97 cells into congenitally athymic mice induced the formation of solid tumor masses that could be retransplanted every 4 weeks. The cells obtained from tumor mass when cultivated in vitro had a morphology comparable to those of the initial culture. 4. This cell line may prove useful for cellular and molecular studies as well as in studies of gliomas treatment.
Subject: glioblastoma
brain neoplasm
human glioma cell lines
Country: EUA
Editor: Kluwer Academic/plenum Publ
Citation: Cellular And Molecular Neurobiology. Kluwer Academic/plenum Publ, v. 21, n. 4, n. 421, n. 428, 2001.
Rights: fechado
Identifier DOI: 10.1023/A:1012662423863
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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