Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/65575
Type: Artigo de periódico
Title: Escherichia coli strains from edema disease: O serogroups, and genes for Shiga toxin, enterotoxins, and F18 fimbriae
Author: da Silva, AS
Valadares, GF
Penatti, MPA
Brito, BG
Leite, DD
Abstract: The objectives of the research were to determine the presence of the gene sequences for Shiga Toxin 2e (Stx2e). enterotoxins (ST-I, ST-II and LT-I), and F18 fimbriae in 144 Escherichia coli strains isolated from pigs with edema disease; to assess the ability of stx2e(+) strains to produce Stx2e; and to determine the O serogroups of the E. coli strains. Presence of the genes was determined by polymerase chain reaction (PCR), production of Stx2e was assessed by cytotoxicity for Vero and Hela cells, O serogroups were identified by agglutination with specific antisera. Of the 144 strains tested, 99 were str2e(divided by) by PCR, but only 45 of these were Stx2e(divided by) in the cell culture assays. Among the 99 stx2e(+) strains, PCR detected the genes for F18ab, ST-I, ST-II, LT-I in 76, 40, 31 and 16 strains, respectively. Forty-one of the 99 sxt2e(+) strains belonged to O group 139; the rest did not belong to the classical edema disease O serogroups. It is likely that the enterotoxins, whose genes were detected at high frequency, are responsible for diarrhea seem in pigs with edema disease in Brazil. (C) 2001 Elsevier Science B.V. All rights reserved.
Subject: Escherichia coli
bacteria
pig
edema disease
shiga toxin
PCR
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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