Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/655
Type: Artigo de periódico
Title: Arginine methylation analysis of the splicing-associated SR protein SFRS9/SRP30C
Author: BRESSAN, Gustavo C.
MORAES, Eduardo C.
MANFIOLLI, Adriana O.
KUNIYOSHI, Tais M.
PASSOS, Dario O.
GOMES, Marcelo D.
KOBARG, Joerg
Abstract: The human SFRS9/SRp30c belongs to the SR family of splicing regulators. Despite evidence that members of this protein family may be targeted by arginine methylation, this has yet to be experimentally addressed. In this study, we found that SFRS9 is a target for PRMT1-mediated arginine methylation in vitro, and that it is immunoprecipitated from HEK-293 lysates by antibodies that recognize both mono- and dimethylated arginines. We further observed that upon treatment with the methylation inhibitor Adox, the fluorescent EGFP-SFRS9 re-localizes to dot-like structures in the cell nucleus. In subsequent confocal analyses, we found that EGFP-SFRS9 localizes to nucleoli in Adox-treated cells. Our findings indicate the importance of arginine methylation for the subnuclear localization of SFRS9.
Subject: Nuclear bodies
Speckles
RGG boxes
Arginine methylation
Protein-protein interaction
Country: Polônia
Editor: VERSITA
Citation: CELLULAR & MOLECULAR BIOLOGY LETTERS, v.14, n.4, p.657-669, 2009
Rights: fechado
Identifier DOI: 10.2478/s11658-009-0024-2
Address: http://apps.isiknowledge.com/InboundService.do?Func=Frame&product=WOS&action=retrieve&SrcApp=EndNote&UT=000269947600008&Init=Yes&SrcAuth=ResearchSoft&mode=FullRecord
http://dx.doi.org/10.2478/s11658-009-0024-2
Date Issue: 2009
Appears in Collections:IB - Artigos e Materiais de Revistas Científicas

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