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Type: Artigo de periódico
Title: Electroanalytical determination of acid phosphatase activity by monitoring p-nitrophenol
Author: Calvo-Marzal, P
Rosatto, SS
Granjeiro, PA
Aoyama, H
Kubota, LT
Abstract: The development of a modified electrode for the selective determination of p-nitrophenol (PNP) to be applied in the determination of the enzymatic activity (EA) of acid phosphatase using p-nitrophenylphosphate (PNPP) as substrate is described. Elimination of the interference of PNPP was made by covering the electrode surface using 25 mul of a 5% (m/v) Nafion (R) solution. In comparison to the glassy carbon (GC) electrode without modification, the presence of the membrane promoted a reduction in the sensitivity and an increase in the detection limit for the determination of PNP, in addition to a decrease of the electron transfer constant. The GC electrode covered with a Nafion (R) membrane showed a linear response range between 20 and 230 mu mol l(-1) for PNP, adjusted by the equation I-p = 0.157 + 0.0089 [PNP], for N = 22 with a correlation coefficient of 0.9984. This electrode was used for the determination of the EA of acid phosphatase from castor bean seeds and an activity of 38.40 U mg(-1) was found. (C) 2001 Elsevier Science B.V. All rights reserved.
Subject: p-nitrophenol
modified glassy carbon electrode
Nafion (R)
acid phosphatase enzyme
Country: Holanda
Editor: Elsevier Science Bv
Citation: Analytica Chimica Acta. Elsevier Science Bv, v. 441, n. 2, n. 207, n. 214, 2001.
Rights: fechado
Identifier DOI: 10.1016/S0003-2670(01)01120-5
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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