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Type: Artigo de periódico
Title: Neutralization of the pharmacological effects of bothropstoxin-I from Bothrops jararacussu (jararacucu) venom by crotoxin antiserum and heparin
Author: Oshima-Franco, Y
Leite, GB
Silva, GH
Cardoso, DF
Hyslop, S
Giglio, JR
da Cruz-Hofling, MA
Rodrigues-Simioni, L
Abstract: Bothropstoxin-I (BthTX-1), the principal myotoxin of Bothrops jararacussu venom, is devoid of phospholipase A(2) (PLA(2)) activity but capable of blocking neuromuscular transmission in mouse nerve-muscle preparations. In this study, the ability of crotoxin antiserum and heparin in preventing the neurotoxic and myotoxic effects of BthTX-I was investigated. Phrenic nerve-diaphragm preparations (PND) stimulated indirectly with supramaximal stimuli (0.2 ms, 0.1 Hz) were incubated with BthTX-I (20 mug/ml) alone or with BthTX-I preincubated with antiserum or heparin for 30 min at 37 degreesC prior to testing. Control preparations were incubated with Tyrode solution, antiserum or heparin alone. BthTX-I (20 mug/ml) produced 50% neuromuscular blockade in the PND preparations in 31 +/- 4 min, with complete blockade occurring in 120 min. The antiserum and heparin significantly prevented the neuromuscular blockade caused by BthTX-I (84 +/- 4% and 100% protection, respectively). Light microscopy examination of the muscles at the end of the 120 min incubation showed that BthTX-I damaged 48 +/- 6% of the fibers. Preincubating the toxin with antivenom significantly reduced the extent of this damage (only 15 +/- 4% of fibers affected, corresponding to 69% protection, P < 0.01) whereas heparin offered no protection (34 +/- 7% of fibers affected, not significantly different from that seen with toxin alone). These results show that the antivenom was more effective in neutralizing the myotoxic effects of BthTX-I than was heparin. (C) 2001 Elsevier Science Ltd. All rights reserved.
Subject: bothropstoxin-I
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Citation: Toxicon. Pergamon-elsevier Science Ltd, v. 39, n. 10, n. 1477, n. 1485, 2001.
Rights: fechado
Identifier DOI: 10.1016/S0041-0101(01)00107-6
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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