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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleNeurotoxic and myotoxic actions of crotoxin-like and Crotalus durissus cascavella whole venom in the chick biventer cervicis preparationpt_BR
dc.contributor.authorBeghini, DGpt_BR
dc.contributor.authorRodrigues-Simioni, Lpt_BR
dc.contributor.authorToyama, MHpt_BR
dc.contributor.authorNovello, JCpt_BR
dc.contributor.authorda Cruz-Hofling, MApt_BR
dc.contributor.authorMarangoni, Spt_BR
unicamp.author.emailhofling@unicamp.brpt_BR
unicamp.authorUniv Estadual Campinas, UNICAMP, Inst Biol, Dept Histol & Embryol, BR-13083970 Campinas, SP, Brazil Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Bioquim, BR-13083970 Campinas, SP, Brazil Univ Estadual Campinas, UNICAMP, Fac Ciencias Med, Dept Farmacol, BR-13083970 Campinas, SP, Brazilpt_BR
dc.subjectneuromuscular blockadept_BR
dc.subjectacetylcholinept_BR
dc.subjectKCIpt_BR
dc.subjectneurotoxicitypt_BR
dc.subjectmyotoxicitypt_BR
dc.subject.wosSouth-american Rattlesnakept_BR
dc.subject.wosNeuromuscular-junctionpt_BR
dc.subject.wosBiological-activitiespt_BR
dc.subject.wosTerrificus Envenomationpt_BR
dc.subject.wosSnake-venomspt_BR
dc.subject.wosComplexpt_BR
dc.subject.wosMusclept_BR
dc.subject.wosToxinpt_BR
dc.subject.wosComponentpt_BR
dc.subject.wosNervept_BR
dc.description.abstractCrotoxin from Crotalus durissus cascavella venom was purified by a combination of molecular exclusion chromatography (Superdex 75 column) and HPLC molecular exclusion (Protein Pack 300SW column). Neurotoxic and myotoxic effects from C. durissus cascavella whole venom and its main fraction, the crotoxin-like, were studied in the chick biventer cervicis (CBC nerve-muscle preparation. Both venom and its crotoxin showed significant (1) < 0.05) blockade of neuromuscular transmission at concentrations as low as 0.2-1, 5 and 25 mug/ml, but no significant effect has been shown with a concentration of 0.04 mug/ml (n = 5 each). The time required to produce 50% neuromuscular blockade with the venom and its crotoxin was 53.6 +/- 8.2 and 65.9 +/- 4.9 min (0.2 mug/ml), 29.7 +/- 1.9 and 34.3 +/- 1.9 min (1 mug/ml), 24.8 +/- 1.6 and 21.1 +/- 1.5 min (5 mug/ml), 20.9 +/- 3.7 and 20.1 +/- 1.4 min (25 mug/ml), respectively. The addition to the incubation bath of acetylcholine (55 and 110 muM) or KC1 (20.1 mM), either before or after the venom or the crotoxin induced contracture in the presence of a total blockade, in all the concentrations used. Morphological analysis showed that the damage caused by C. durissus cascavella venom is stronger than that caused by crotoxin. The myonecrotic picture was more marked at higher venom and crotoxin doses (4, 5 or 25 mug/ml). Only at 25 mug/ml concentrations of the venom and crotoxin, marked muscle fiber changes were detected. We concluded that the crotoxin-like and the whole venom from C. durissus cascavella possess a preponderant and quite potent neurotoxic action in this preparation, and a myotoxic action which is observed only at higher doses. (C) 2004 Elsevier Ltd. All rights reserved.pt
dc.relation.ispartofToxiconpt_BR
dc.relation.ispartofabbreviationToxiconpt_BR
dc.publisher.cityOxfordpt_BR
dc.publisher.countryInglaterrapt_BR
dc.publisherPergamon-elsevier Science Ltdpt_BR
dc.date.issued2004pt_BR
dc.date.monthofcirculation36951pt_BR
dc.identifier.citationToxicon. Pergamon-elsevier Science Ltd, v. 43, n. 3, n. 255, n. 261, 2004.pt_BR
dc.language.isoenpt_BR
dc.description.volume43pt_BR
dc.description.issuenumber3pt_BR
dc.description.firstpage255pt_BR
dc.description.lastpage261pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policypt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0041-0101pt_BR
dc.identifier.wosidWOS:000221021900004pt_BR
dc.identifier.doi10.1016/j.toxicon.2003.12.001pt_BR
dc.date.available2014-11-19T10:11:45Z
dc.date.available2015-11-26T17:05:00Z-
dc.date.accessioned2014-11-19T10:11:45Z
dc.date.accessioned2015-11-26T17:05:00Z-
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dc.description.provenanceMade available in DSpace on 2015-11-26T17:05:00Z (GMT). No. of bitstreams: 2 WOS000221021900004.pdf: 272077 bytes, checksum: 682758351aa599a026d9a705abe32def (MD5) WOS000221021900004.pdf.txt: 25651 bytes, checksum: 7c129f36dc401ebfd98ce190bf3d372c (MD5) Previous issue date: 2004en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/65313pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/65313
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/65313-
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