Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/65294
Type: Artigo de periódico
Title: Neuromuscular and phospholipase activities of venoms from three subspecies of Bothrops neuwiedi (B-n. goyazensis, B-n. paranaensis and B-n. diporus)
Author: Abreu, VA
Belo, CAD
Hernandes-Oliveira, SS
Borja-Oliveira, CR
Hyslop, S
Furtado, MDFD
Rodrigues-Simioni, L
Abstract: The Bothrops neuwiedi (Neuwied's lancehead) species complex consists of a variety of subspecies with a wide distribution in South America. In this work, we compared the neuromuscular blockade caused by venoms from three subspecies (B. n. goyazensis, B. n. paranaensis and B. n. diporus) of this complex using chick biventer cervicis (BC) and mouse phrenic nerve-diaphragm (PND) preparations and investigated their phospholipase A, (PLA(2)) activities and electrophoretic profiles. The order of potency of PLA2 activity was B. n. diporus > B. n. paranaensis > B. n. goyazensis. In BC preparations, B. n. goyazensis venom (50 mu g/mL) was significantly (p < 0.05) more active than B. n. paranaensis and B. n. diporus venoms, which did not produce a significant blockade at this time interval;, after 120 min, B. n. goyazensis, B. n. paranaensis and B. n. diporus venoms (100 mu g/mL) produced blockades of 57.4 +/- 5%, 30 +/- 3% and 17.4 +/- 7% (n=3-6 each), respectively. The three venoms inhibited contractures in response to ACh, indicating interference with postsynaptic neurotransmission. Only B. n. goyazensis and B. n. paranaensis venoms caused a longlasting, concentration-dependent muscle contracture prior to blockade. In PND preparations, all of the venoms blocked the twitch-tension responses within 45-100 min, indicating that these preparations were more sensitive than avian preparations. There was a correlation between PLA2 activity and the time for 50% blockade in PND but not in BC preparations. SDS-PAGE showed quantitative rather than qualitative differences among the venoms. These results indicate that the venoms of the three subspecies had similar profiles of neuromuscular activity, although the relationship with PLA(2) activity varied with the preparation used. (c) 2007 Elsevier Inc. All rights reserved.
Subject: Bothrops
chick biventer cervicis
mouse phrenic nerve-diaphragm
myotoxins
neuromuscular junction
phospholipase A(2)
snake venoms
Country: EUA
Editor: Elsevier Science Inc
Rights: fechado
Identifier DOI: 10.1016/j.cbpa.2007.03.030
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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