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Type: Artigo de periódico
Title: Neuromuscular action of venom from the South American colubrid snake Philodryas patagoniensis
Author: da Costa, RSC
Prudencio, L
Ferrari, EF
Souza, GHMF
de Mello, SM
Prianti, ACG
Ribeiro, W
Zamuner, SR
Hyslop, S
Cogo, JC
Abstract: Snakes of the opisthoglyphous genus Philodryas are widespread in South America and cause most bites by colubrids in this region. In this study, we examined the neurotoxic and myotoxic effects of venom from Philodtyas patagoniensis in biventer cervicis and phrenic nerve-diaphragm preparations and we compared the biochemical activities of venoms from P. patagoniensis and Philodryas olfersii. Philodryas patagoniensis venom (40 mu g/mL) had no effect on mouse phrenic nerve-diaphragm preparations but caused time-dependent neuromuscular blockade of chick biventer cervicis preparations. This blockade was not reversed by washing. The highest concentration of venom tested (40 mu g/mL) significantly reduced (p < 0.05) the contractures to exogenous acetylcholine (55 AM and 110 mu M) and K+(13.4 mM) after 120 min; lower concentrations of venom had no consistent or significant effect on these responses. Venom caused a concentration- and time-dependent release of creatine kinase (CK) from biventercervicis preparations. Histological analysis showed contracted muscle fibers at low venom concentrations and myonecrosis at high concentrations. Philodryas venoms had low esterase and phospholipase A(2) but high proteolytic activities compared to the pitviper Bothrops jararaca. SDS-PAGE showed that the Philodryas venoms had similar electrophoretic profiles, with most proteins having a molecular mass of 25-80 kDa. Both of the Philodryas venoms cross-reacted with bothropic antivenom in ELISA, indicating the presence of proteins immunologically related to Bothrops venoms. RP-HPLC of P. patagoniensis venom yielded four major peaks, each of which contained several proteins, as shown by SDS-PAGE. These results indicate that P. patagoniensis venom has neurotoxic and myotoxic components that may contribute to the effects of envenoming by this species. (c) 2008 Elsevier Inc. All rights reserved.
Subject: antivenom
neuromuscular effects
Philodryas putagoniensis
Country: EUA
Editor: Elsevier Science Inc
Citation: Comparative Biochemistry And Physiology C-toxicology & Pharmacology. Elsevier Science Inc, v. 148, n. 1, n. 31, n. 38, 2008.
Rights: fechado
Identifier DOI: 10.1016/j.cbpc.2008.03.006
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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