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Type: Artigo de periódico
Title: Electrocoagulation as a Clarifiation and Pre-purification Step in Plant Based Bioprocesses
Author: Robic, G
Miranda, EA
Abstract: Electrocoagulation is a technique that has been applied to water and wastewater treatment. We propose here extension of this technique to the field of plant downstream processing (DSP), where plants are used as a bioreactors for recombinant protein production. The main problem in plant-based bioreactors is the presence of chlorophyll and phenolic compounds in plant extracts, which tend to precipitate and denature proteins. Their removal from the extracts is essential, since they have a large impact on the DSP performance. In the present work we studied the application of an electrocoagulation based technique to clarify tobacco leaf extracts by removing chlorophyll and phenolic compounds. By manipulating the pH of electrocoagulation, 90% of chlorophyll, 65% of phenolic compounds, and only 23% of total protein were removed from the extract. The process preferentially removes proteins with more acidic pI (below 6.03) and the pH of the process differentially effects removal of acidic and basic proteins.
Subject: chlorophyll
phenolic compounds
aluminium hydroxide
Country: Coreia do Sul
Editor: Korean Soc Biotechnology & Bioengineering
Citation: Biotechnology And Bioprocess Engineering. Korean Soc Biotechnology & Bioengineering, v. 16, n. 4, n. 777, n. 784, 2011.
Rights: fechado
Identifier DOI: 10.1007/s12257-010-0420-5
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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