Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/65101
Full metadata record
DC FieldValueLanguage
dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleEffects of stretching on morphological and biochemical aspects of the extracellular matrix of the rat calcaneal tendonpt_BR
dc.contributor.authorde Almeida, FMpt_BR
dc.contributor.authorTomiosso, TCpt_BR
dc.contributor.authorBiancalana, Apt_BR
dc.contributor.authorMattiello-Rosa, SMpt_BR
dc.contributor.authorVidal, BCpt_BR
dc.contributor.authorGomes, Lpt_BR
dc.contributor.authorPimentel, ERpt_BR
unicamp.author.emailpimentel@unicamp.brpt_BR
unicamp.authorde Almeida, Fernanda Martins Tomiosso, Tatiana Carla Biancalana, Adriano Pimentel, Edson Rosa Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Anat Biol Celular & Fisiol Biofis, BR-13083863 Campinas, SP, Brazilpt_BR
unicamp.authorVidal, Benedicto Campos Gomes, Laurecir Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Biol Celular, BR-13083863 Campinas, SP, Brazilpt_BR
unicamp.authorde Almeida, Fernanda Martins Univ Fed Rio de Janeiro, Inst Cencias Biomed, Rio De Janeiro, Brazilpt_BR
unicamp.authorTomiosso, Tatiana Carla Univ Fed Fluminense, Dept Interdisciplinar PURO, Rio Das Ostras, RJ, Brazilpt_BR
unicamp.authorMattiello-Rosa, Stela Marcia Univ Fed Sao Carlos, Dept Fisioterapia, BR-13565905 Sao Carlos, SP, Brazilpt_BR
dc.subjectStretchingpt_BR
dc.subjectTendonpt_BR
dc.subjectBiochemistrypt_BR
dc.subjectMorphologypt_BR
dc.subjectRat (Wistar)pt_BR
dc.subject.wosBovine Tendonpt_BR
dc.subject.wosGlycosaminoglycan Contentpt_BR
dc.subject.wosBiomechanical Propertiespt_BR
dc.subject.wosPhysical-exercisept_BR
dc.subject.wosAchilles-tendonpt_BR
dc.subject.wosFlexor Tendonpt_BR
dc.subject.wosAging Ratspt_BR
dc.subject.wosCollagenpt_BR
dc.subject.wosProteoglycanspt_BR
dc.subject.wosCartilagept_BR
dc.description.abstractSeveral studies have demonstrated the relationship between exercise and the extracellular matrix of muscle tendons, and have described alterations in their structural and biochemical properties when subjected to strenuous exercise. However, little is known about what happens to tendons when they are subjected to stretching. We evaluated the changes in the composition and structure of rat calcaneal tendons subjected to a stretching program. The animals had their muscles stretched for 30 s with 30 s of rest, with 10 repetitions, three and five times a week for 21 days. For morphological analysis, the sections were stained with hematoxylin-eosin and toluidine blue. For biochemical analysis, the tendons were treated with 4 M guanidine hydrochloride and analyzed in SDS-PAGE. The contents of total proteins and glycosaminoglycans were also measured. In the sections stained with toluidine blue, we could observe an increase of rounded cells, especially in the enthesis region. In the region next to the enthesis was a metachromatic region, which was more intensely stained in the stretched groups. In the tension regions, the cells appeared more aligned. Cellularity increased in both regions. The SDS-PAGE analysis showed a larger amount of collagen in the stretched groups and a polydispersed component of 65 kDa in all the groups. The amounts of proteins and glycosaminoglycans were also larger in the stretched tendons. The agarose-gel electrophoresis confirmed the presence of dermatan sulfate in the tension and compression regions, and of chondroitin sulfate only in the latter. Our results showed that the stretching stimulus changed the cellularity and the amount of the extracellular matrix compounds, confirming that tendons are dynamic structures with a capacity to detect alterations in their load.pt
dc.relation.ispartofCell And Tissue Researchpt_BR
dc.relation.ispartofabbreviationCell Tissue Res.pt_BR
dc.publisher.cityNew Yorkpt_BR
dc.publisher.countryEUApt_BR
dc.publisherSpringerpt_BR
dc.date.issued2010pt_BR
dc.date.monthofcirculationOCTpt_BR
dc.identifier.citationCell And Tissue Research. Springer, v. 342, n. 1, n. 97, n. 105, 2010.pt_BR
dc.language.isoenpt_BR
dc.description.volume342pt_BR
dc.description.issuenumber1pt_BR
dc.description.firstpage97pt_BR
dc.description.lastpage105pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0pt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0302-766Xpt_BR
dc.identifier.wosidWOS:000282428200010pt_BR
dc.identifier.doi10.1007/s00441-010-1027-zpt_BR
dc.date.available2014-11-18T17:19:32Z
dc.date.available2015-11-26T16:57:29Z-
dc.date.accessioned2014-11-18T17:19:32Z
dc.date.accessioned2015-11-26T16:57:29Z-
dc.description.provenanceMade available in DSpace on 2014-11-18T17:19:32Z (GMT). No. of bitstreams: 1 WOS000282428200010.pdf: 692363 bytes, checksum: 2d0bc174bcdd0b8269b3e72c236cf2a9 (MD5) Previous issue date: 2010en
dc.description.provenanceMade available in DSpace on 2015-11-26T16:57:29Z (GMT). No. of bitstreams: 2 WOS000282428200010.pdf: 692363 bytes, checksum: 2d0bc174bcdd0b8269b3e72c236cf2a9 (MD5) WOS000282428200010.pdf.txt: 36507 bytes, checksum: cfa74e49ab238c98716c0f0448aa864c (MD5) Previous issue date: 2010en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/65101pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/65101
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/65101-
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
WOS000282428200010.pdf676.14 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.