Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/65028
Type: Artigo de periódico
Title: Effects of neonatal capsaicin treatment in the neutrophil production, and expression of preprotachykinin-I and tachykinin receptors in the rat bone marrow
Author: Franco-Penteado, CF
De Souza, IA
Lima, CSP
Teixeira, SA
Muscara, MN
De Nucci, G
Antunes, E
Abstract: Bone marrow is richly innervated with both myelinated and non-myelinated nerve fibers, but the role of this innervation on hemopoiesis is poorly understood. Therefore, the aim of this study was to investigate the role of C-fibers on hernatopoiesis. Wistar rats were neonatally injected with either capsaicin or its vehicle, and used at adult ages (8-10 weeks). In capsaicin-pretreated rats, the levels of substance P (SP) in bone marrow fluid were markedly reduced in comparison with the vehicle group (13.1 +/- 4.5 pg/ml versus 47.3 +/- 5.5 pg/ml, p < 0.05). In bone marrow, the number of total leukocytes was 28% higher (p < 0.05) in capsaicin-pretreated group, and this accompanied by a higher number of neutrophils, particularly of the immature forms. The mononuclear cell and eosinophils counts did not differ significantly among vehicle and capsaicin groups. In peripheral blood, the number of circulating neutrophils in the capsaicin group increased by 53.8% (p < 0.05), whereas the number of mononuclear cells did not change significantly among groups. Eosinophils were virtually absent in the circulating blood in both groups. Semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) showed that both preprotachykinin (PPT)-I mRNA and the tachykinin neurokinin (NK)-1 mRNA expression in bone marrow cells significantly increased in capsaicin group, whereas the NK-2 mRNA expression was unchanged after capsaicin pretreatment. In conclusion, our data show that chronic neuropeptide depletion enhance the neutrophil proliferation and differentiation in the rat bone marrow by mechanisms involving upregulation of PPT-I gene and NK-1 receptors. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
Subject: neuropeptides
bone marrow
neutrophils
capsaicin
hematopoiesis
Country: Irlanda
Editor: Elsevier Ireland Ltd
Rights: fechado
Identifier DOI: 10.1016/j.neulet.2006.08.002
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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