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Type: Artigo de periódico
Title: Enemas with n-Acetylcysteine Can Reduce the Level of Oxidative Damage in Cells of the Colonic Mucosa Diverted from the Faecal Stream
Author: Martinez, CAR
de Almeida, MG
da Silva, CMG
Ribeiro, ML
da Cunha, FL
Rodrigues, MR
Sato, DT
Pereira, JA
Abstract: Oxidative stress has been related to inflammation of the colonic mucosa in patients with diversion colitis (DC). The purpose of this study was to evaluate the antioxidants effects of n-acetylcysteine (NAC) in colon segments without faecal stream. Thirty-six Wistar rats were subjected to diversion of the faecal stream by proximal colostomy and a distal mucosal colon fistula. They were distributed into three experimental groups of 12 animals each; the animals in each group underwent daily enemas containing saline solution (control group) or either a 25 or 100 mg/kg dose of NAC (treated groups). In each group, animals were sacrificed after 2 or 4 weeks. The degree of inflammation was determined by histopathological analysis and stratified by inflammatory grading scale. Oxidative DNA damage was measured by comet assay. The Mann-Whitney test and ANOVA were used for statistical analysis; p < 0.05 was considered significant. The oxidative DNA damage in colon segments without faecal stream was significantly lower in animals treated with either concentration of NAC than in control group, regardless of the duration of intervention (p < 0.01). Intrarectal application of NAC reduces the inflammation as well as DNA oxidative damage and could be beneficial as a complementary agent in the treatment of DC.
Subject: n-Acetylcysteine
Reactive oxygen species
Oxidative stress
Comet assay
Short-chain fatty acids
Country: Holanda
Editor: Springer
Citation: Digestive Diseases And Sciences. Springer, v. 58, n. 12, n. 3452, n. 3459, 2013.
Rights: fechado
Identifier DOI: 10.1007/s10620-013-2768-9
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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