Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/64778
Type: Artigo de periódico
Title: Endotoxin removal from solutions of F(ab ')(2) fragments of equine antibodies against snake venom using macroporous chitosan membrane
Author: de Freitas, SS
Machado, RL
de Arruda, EJ
Santana, CC
Bueno, SMA
Abstract: The adsorptive filtration technique using chitosan membranes was used to remove endotoxin (ET) from buffers and bothrops antivenom serum (F(ab')(2) fragments of equine antibodies). Macroporous chitosan membrane was prepared from chitosan using silica particles as porogen agent. The effects of solution conditions on the efficiency of endotoxin removal and protein recovery were studied. The ET concentrations were reduced to 0.58 and 71.6 EU/ml, respectively, when Tris-HCl pH 7.0 buffer with 18.6 and 8092.5 EU/ml was passed through the chitosan membrane. This buffer was used to evaluate ET removal in the presence of the equine F(ab')(2) fragments. The results showed that the presence of protein in the feed solution did not influence much the clearance of this membrane. Removal of 97% of the ET and recovery of 94 + of the F(ab')2 were obtained at an initial ET and F(ab')(2) concentration of 974.4 EU/ml and 18.6 mg/ml, respectively. (C) 2004 Elsevier B.V. All rights reserved.
Subject: depyrogenation
endotoxins
snake antivenom
chitosan
adsorptive membranes
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Identifier DOI: 10.1016/j.memsci.2003.12.019
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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