Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/64765
Type: Artigo de periódico
Title: Endosperm-preferred expression of maize genes as revealed by transcriptome-wide analysis of expressed sequence tags
Author: Verza, NC
Silva, TRE
Neto, GC
Nogueira, FTS
Fisch, PH
de Rosa, VE
Rebello, MM
Vettore, AL
da Silva, FR
Arruda, P
Abstract: The transcriptome-wide endosperm-preferred expression of maize genes was addressed by analyzing a large database of expressed sequence tags (ESTs). We generated 30,531 high quality sequence-reads from the 5'-ends of cDNA libraries from maize endosperm harvested at 10, 15, and 20 days after pollination. A further 196,900 maize sequence-reads retrieved from public databases were added to this endosperm collection to generate MAIZEST, a database with tools for data storage and analysis. MAIZEST contains 227,431 ESTs, one third of which represents developing endosperm and the remaining two-thirds represent transcripts from 49 cDNA libraries constructed from different organs and tissues. Assembling the MAIZEST ESTs generated 29,206 putative transcripts, of which a set of 4032 assembled sequences was composed exclusively of sequences derived from endosperm cDNA libraries. After sequence analysis using overlapping parameters, a sub-set of 2403 assembled sequences was functionally annotated and revealed a wide variety of putative new genes involved in endosperm development and metabolism.
Subject: endosperm
endosperm-specific genes
ESTs
maize transcriptome
Country: Holanda
Editor: Springer
Rights: fechado
Identifier DOI: 10.1007/s11103-005-8924-7
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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