Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/64748
Type: Artigo de periódico
Title: Endogenous lectin as a possible regulator of the hydrolysis of physiological substrates by soybean seed acid phosphatase
Author: Aoyama, H
Cavagis, ADM
Taga, EM
Ferreira, CV
Abstract: The effects of two lectins concanavalin A (conA) and soybean agglutinin, on soybean seed acid phosphatase activity were investigated using p-nitrophenylphosphate (pNPP), pyrophosphate (PPi) and phosphoenolpyruvate (PEP) as substrates. Of the four acid phosphatase isoforms (AP1, AP2, AP3A and AP3B) purified from soybean seeds, only API was activated 40 and 60% by conA and soybean agglutinin, respectively. Both lectins affected some of the kinetic parameters of AP1. The activation by lectins was not affected by 1 mM Ca2+ or Mn2+ but glucose and methylmannopyranoside (100 mM) prevented activation by conA. Under the same conditions, galactose had no effect. These results suggest that plant acid phosphatases may be regulated by lectins, the effects vary according to the substrate used. (C) 2001 Elsevier Science Ltd. All rights reserved.
Subject: Glycine max
leguminosae
soybean agglutinin
concanavalin A
soybean seed
acid phosphatase
lectins
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Rights: fechado
Identifier DOI: 10.1016/S0031-9422(01)00190-X
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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