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Type: Artigo de periódico
Title: Elevation of resting mitochondrial membrane potential of neural cells by cyclosporin A, BAPTA-AM, and Bcl-2
Author: Kowaltowski, AJ
Smaili, SS
Russell, JT
Fiskum, G
Abstract: This study tested the hypothesis that the activity of the mitochondrial membrane permeability transition pore (PTP) affects the resting mitochondrial membrane potential (Delta Psi) of normal, healthy cells and that the anti-apoptotic gene product Bcl-2 inhibits the basal activity of the PTP. Delta Psi was measured by both fluorometric and nonfluorometric methods with SY5Y human neuroblastoma cells and with GT1-7 hypothalamic cells and PC12 pheochromocytoma cells in the absence and presence of Bcl-2 gene overexpression. The resting Delta Psi of Bcl-2 nonexpressing PC12 and wild-type SY5Y cells was increased significantly by the presence of the PTP inhibitor cyclosporin A (CsA) or by intracellular Ca2+ chelation through exposure to the acetoxymethyl ester of 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA-AM). The Delta Psi of Bcl-2-overexpressing PC12 cells was larger than that of Bcl-2-negative cells and not significantly increased by CsA or by Ca2+ chelation. CsA did not present a significant effect on the Delta Psi monitored in unstressed GT1-7 cells but did inhibit the decrease in Delta Psi elicited by the addition of t-butyl hydroperoxide, an oxidative inducer of the mitochondrial permeability transition. These results support the hypothesis that an endogenous PTP activity can contribute to lowering the basal Delta Psi of some cells and that Bcl-2 can regulate the endogenous activity of the mitochondrial PTP.
Subject: calcium
mitochondrial permeability transition
energy metabolism
Country: EUA
Editor: Amer Physiological Soc
Citation: American Journal Of Physiology-cell Physiology. Amer Physiological Soc, v. 279, n. 3, n. C852, n. C859, 2000.
Rights: embargo
Date Issue: 2000
Appears in Collections:Unicamp - Artigos e Outros Documentos

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