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|Type:||Artigo de periódico|
|Title:||ELECTROTRANSFORMATION OF 3 PATHOVARS OF XANTHOMONAS-CAMPESTRIS|
|Abstract:||Procedures for electrotransformation have been adapted for three pathovars of Xanthomonas campestris: campestris, vesicatoria and manihotis. Three differently sized plasmids (51, 9.3 and 3.3 kb) at different concentrations (10, 30 and 50 ng/sample) and different field strengths (10, 12, 14 and 18 kV/cm) were used. The efficiency of transformation was dependent on the recipient strain and the plasmid introduced. In general, a field strength of 14 kV/cm as well as a concentration of 30 ng plasmid DNA/sample seemed to be adequate for most conditions. Only X, campestris pv. vesicatoria strain 479 required a higher field strength for better efficiency. The plasmid size was inversely related to the efficiency of transformation; up to 1.6 x 10(10), 5.3 x 10(7) and 3.7 x 10(5) transformants/mu g DNA were obtained using the pXG31 (3.3 kb), pUFR027 (9.3 kb) and RP4 (51 kb) plasmids, respectively.|
|Citation:||Applied Microbiology And Biotechnology. Springer Verlag, v. 43, n. 4, n. 651, n. 655, 1995.|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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