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Type: Artigo de periódico
Title: Electrospray ionization mass spectrometry analysis of polyisoprenoid alcohols via Li+ cationization
Author: D'Alexandri, FL
Gozzo, FC
Eberlin, MN
Katzin, AM
Abstract: Direct analysis of polyisoprenoids by electrospray ionization mass spectrometry (ESI-MS) often produces poor results requiring offline time and sample-consuming derivatization techniques. We describe a simple ESI-MS approach for the direct analysis of polyisoprenoids using several dolichols and polyprenols with different chain sizes as proof-of-principle cases. Lithium iodide is used to promote cationization by intense formation of [M + Li](+) adducts. Thus, detection of polyisoprenoids with mass determination can be performed with high sensitivity (limit of detection [LOD] similar to 100 rho M), whereas characteristic collision-induced dissociations observed for both dolichols and polyprenols permit investigation of their structure. Using ESI(Li+)-MS and ESI(Li+)-MS/MS analysis, we screened for polyprenol products of an octaprenyl pyrophosphate synthase of Plasmodium falciparum and dolichols in a complex mixture of compounds produced by Leishmania amazonensis and P. falciparum. (c) 2006 Elsevier Inc. All rights reserved.
Subject: mass spectrometry
Plasmodium falciparum
Leishmania amazonensis
Country: EUA
Editor: Academic Press Inc Elsevier Science
Citation: Analytical Biochemistry. Academic Press Inc Elsevier Science, v. 355, n. 2, n. 189, n. 200, 2006.
Rights: fechado
Identifier DOI: 10.1016/j.ab.2006.06.014
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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