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|Type:||Artigo de periódico|
|Title:||Electrospray ionization mass spectrometry analysis of polyisoprenoid alcohols via Li+ cationization|
|Abstract:||Direct analysis of polyisoprenoids by electrospray ionization mass spectrometry (ESI-MS) often produces poor results requiring offline time and sample-consuming derivatization techniques. We describe a simple ESI-MS approach for the direct analysis of polyisoprenoids using several dolichols and polyprenols with different chain sizes as proof-of-principle cases. Lithium iodide is used to promote cationization by intense formation of [M + Li](+) adducts. Thus, detection of polyisoprenoids with mass determination can be performed with high sensitivity (limit of detection [LOD] similar to 100 rho M), whereas characteristic collision-induced dissociations observed for both dolichols and polyprenols permit investigation of their structure. Using ESI(Li+)-MS and ESI(Li+)-MS/MS analysis, we screened for polyprenol products of an octaprenyl pyrophosphate synthase of Plasmodium falciparum and dolichols in a complex mixture of compounds produced by Leishmania amazonensis and P. falciparum. (c) 2006 Elsevier Inc. All rights reserved.|
|Editor:||Academic Press Inc Elsevier Science|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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