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Type: Artigo de periódico
Title: Effect of nitrofen in the final stages of development of the diaphragm muscle in rats
Author: Goncalves, FLL
de Oliveira, FS
Schmidt, AF
Pereira, LAVD
Gallindo, RM
Figueira, RL
Sbragia, L
Abstract: PURPOSE: To evaluate the expression of myosin in muscle fibers of the diaphragm in experimental congenital diaphragmatic hernia (CDH). METHODS: Fetuses of pregnant rats were divided into four groups: External Control (EC), composed of non-manipulated rats; Nitrofen, composed of pregnant rats that received 100 mg of nitrofen (2,4-dichloro-4' nitrodiphenyl ether) diluted in olive oil on gestational day (GD) 9.5, whose fetuses developed CDH (N+) or not (N-), and Olive Oil Placebo (OO), composed of pregnant rats that received the oil on the same GD. The fetuses were collected on GD 18.5, 19.5, 20.5 and 21.5 (term = 22 days). We obtained body weight (BW) and photographed the diaphragm area (DA), hernia area (HA) and subsequent calculated the HA/DA ratio in N+ group. Samples of Diaphragm muscle were processed for histological staining with H/E and immunohistochemistry (IHQ) for myosin. RESULTS: The fetuses of N- and N+ groups had decreased BW and DA compared to EC and OO groups (p < 0.001). HA was decreased on GD 18.5 compared to 21.5 (p < 0.001) and the HA/DA ratio showed no difference. IHQ showed decreased expression of myosin in nitrofen groups. CONCLUSION: CDH induced by nitrofen model contributes to the understanding of muscularization in the formation of the diaphragm where the myosin expression is decreased.
Subject: Hernia
Abnormalities, Drug-Induced
Country: Brasil
Editor: Acta Cirurgica Brasileira
Citation: Acta Cirurgica Brasileira. Acta Cirurgica Brasileira, v. 28, n. 13, n. 18, 2013.
Rights: aberto
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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