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Type: Artigo de periódico
Title: Effect of poly (ethylene) glycol and albumin on the immobilization of microbial lipase and catalysis in organic media
Author: Soares, CMF
Santana, MHA
Zanin, GM
de Castro, HF
Abstract: Poly (ethylene) glycol (PEG) and bovine serum albumin (BSA), as additive agents, were used to enhance the activity of immobilized microbial lipase in organic solvent. Controlled pore silica (CPS) was selected as matrix and different immobilization procedures were evaluated: directly lipase binding on CPS and simultaneous addition of lipase and additive agent on the same support. The highest coupling yield (59.6%) was attained when the immobilization procedure was performed at lipase loading of 150 U/g support in the presence of PEG-1.500. This immobilized system was used in esterification reactions under repeated batch cycles and the biocatalyst half-life was found to increase 2.7 times when compared with the control.
Subject: additives
controlled pore silica
immobilized lipase
Country: Brasil
Editor: Soc Brasileira Quimica
Citation: Quimica Nova. Soc Brasileira Quimica, v. 26, n. 6, n. 832, n. 838, 2003.
Rights: aberto
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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