Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/64212
Full metadata record
DC FieldValueLanguage
dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleNeonatal exposure to high doses of 17 beta-estradiol results in inhibition of heparanase-1 expression in the adult prostatept_BR
dc.contributor.authorAugusto, TMpt_BR
dc.contributor.authorRosa-Ribeiro, Rpt_BR
dc.contributor.authorCarvalho, HFpt_BR
unicamp.author.emailhern@unicamp.brpt_BR
unicamp.authorAugusto, Taize M. Rosa-Ribeiro, Rafaela Carvalho, Hernandes F. State Univ Campinas UNICAMP, Dept Anat Cell Biol Physiol & Biophys, Inst Biol, BR-13083863 Campinas, SP, Brazilpt_BR
dc.subjectDNA methylationpt_BR
dc.subjectEpigenetic regulationpt_BR
dc.subjectEstrogen imprintingpt_BR
dc.subjectHeparanase-1pt_BR
dc.subjectProstatept_BR
dc.subject.wosRibosomal-rna Genespt_BR
dc.subject.wosRat Ventral Prostatept_BR
dc.subject.wosSulfate Proteoglycanpt_BR
dc.subject.wosMethylationpt_BR
dc.subject.wosEstrogenpt_BR
dc.subject.wosCancerpt_BR
dc.subject.wosAngiogenesispt_BR
dc.subject.wosEstradiolpt_BR
dc.subject.wosReceptorpt_BR
dc.subject.wosCellspt_BR
dc.description.abstractHeparanase-1 (HPSE-1) is an endoglycosidase that cleaves heparan sulfate. The physiological functions of HPSE-1 include embryo development, hair growth, wound healing, tumor growth, angiogenesis, metastasis, and inflammation. HPSE-1 expression was found to increase temporarily in the rat ventral prostate (VP) after castration. The promoter region of the Hpse-1 gene has estrogen-responsive elements, suggesting that the gene is regulated by estrogens. In this study, we investigated the expression of HPSE-1 in the VP of 90-day-old rats after neonatal exposure to a high dose of 17 beta-estradiol. HPSE-1 was not found by immunohistochemistry in the epithelium of estrogenized animals. To determine whether inhibition of Hpse-1 expression in the epithelium was due to pre- or post-transcriptional regulation, epithelial cells were isolated by centrifugation in Percoll gradient and the presence of Hpse-1 mRNA was investigated by RT-PCR. Hpse-1 mRNA was not detected in the estrogenized animals. Considering that Hpse-1 transcription could be inhibited by DNA methylation, we used the methylation-sensitive restriction enzyme HpaII and PCR to show that a single CCGG site at position +185 was more frequently methylated in the epithelium of estrogenized than in control animals. Immunohistochemistry for 5-methylcytidine revealed that the epithelial cell nuclei in estrogenized animals were heavily methylated. These results suggest that Hpse-1 expression was blocked in the epithelial cells of the VP, by estrogen imprinting by a pre-transcriptional mechanism involving DNA methylation.pt
dc.relation.ispartofHistochemistry And Cell Biologypt_BR
dc.relation.ispartofabbreviationHistochem. Cell Biol.pt_BR
dc.publisher.cityNew Yorkpt_BR
dc.publisher.countryEUApt_BR
dc.publisherSpringerpt_BR
dc.date.issued2011pt_BR
dc.date.monthofcirculationNOVpt_BR
dc.identifier.citationHistochemistry And Cell Biology. Springer, v. 136, n. 5, n. 609, n. 615, 2011.pt_BR
dc.language.isoenpt_BR
dc.description.volume136pt_BR
dc.description.issuenumber5pt_BR
dc.description.firstpage609pt_BR
dc.description.lastpage615pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0pt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0948-6143pt_BR
dc.identifier.wosidWOS:000297129900009pt_BR
dc.identifier.doi10.1007/s00418-011-0860-9pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsordocumentnumberFAPESP [09/16150-6]pt
dc.date.available2014-07-30T17:09:03Z
dc.date.available2015-11-26T17:45:32Z-
dc.date.accessioned2014-07-30T17:09:03Z
dc.date.accessioned2015-11-26T17:45:32Z-
dc.description.provenanceMade available in DSpace on 2014-07-30T17:09:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2011en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:45:32Z (GMT). No. of bitstreams: 2 WOS000297129900009.pdf: 1916784 bytes, checksum: db2c7e1194d9dd70cadc48819445fad1 (MD5) WOS000297129900009.pdf.txt: 30446 bytes, checksum: f473f97c6751c01f71ec8f45cec7daeb (MD5) Previous issue date: 2011en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/64212
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/64212-
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
WOS000297129900009.pdf1.87 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.