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Type: Artigo de periódico
Title: Effects of fungal laccase immobilization procedures for the development of a biosensor for phenol compounds
Author: Freire, RS
Duran, N
Kubota, LT
Abstract: Fungal laccase was immobilized on carbon-fiber electrodes using classical methods: physical adsorption, glutaraldehyde, carbodiimide and carbodiimide/glutaraldehyde. The highest biosensor response was obtained using carbodiimide/glutaraldehyde for coupling laccase to carboxyl groups on the carbon fibers. In this method, different percentages of glutaraldehyde had important effects on the sensitivity of the biosensor, the best percentage of glutaraldehyde being 10% (m/v). The behavior of the obtained biosensor was investigated in terms of sensitivity, operational range, pH and applied potential. The developed biosensor showed an optimum response at pH 5.0 and at an applied potential of - 100 mV. The immobilized laccase retained a good activity for over 2 months. (C) 2001 Elsevier Science B.V. All rights: reserved.
Subject: biosensor
carbon fibers
enzyme immobilization
phenolic compounds
Country: Holanda
Editor: Elsevier Science Bv
Citation: Talanta. Elsevier Science Bv, v. 54, n. 4, n. 681, n. 686, 2001.
Rights: fechado
Identifier DOI: 10.1016/S0039-9140(01)00318-6
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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