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|Type:||Artigo de periódico|
|Title:||Effect of therapy with recombinant human interferon-gamma on the release of nitric oxide by neutrophils and mononuclear cells from patients with chronic granulomatous disease|
|Abstract:||The aim of this study was to investigate the effect of recombinant human interferon-gamma (rHuIFN-gamma) therapy on the release of nitric oxide (NO) by neutrophils (NEU) and mononuclear cells (MON) from patients with chronic granulomatous disease (CGD), Five patients with this rare disease received rHuIFN-gamma (50 mu g/m(2) of body surface, given by subcutaneous injection three times a week) for 6 months. Clinical and laboratory evaluations were performed before and after 1 and 6 months of rHuIFN-gamma therapy, Nitric oxide release by NEU and MON was assessed by the ability of these cells to inhibit thrombin-induced washed platelet aggregation, The nitrite (NO2-) and nitrate (NO3-) levels in the supernatant of cultured NEU and MON, as well as in plasma and urine (24 h diuresis), were quantified by high-performance liquid chromatography (HPLC), Conventional immunologic tests for assessing phagocyte and lymphocyte functions and humoral immunity were also performed, Therapy with rHuIFN-gamma for 6 months did not enhance NO synthesis by NEU or MON from the patients with CGD, The urinary but not plasma levels of NO2- and NO3- were elevated after rHuIFN-gamma therapy, Phagocyte and lymphocyte functions as well as humoral immunity were not affected by rHuIFN-gamma therapy, Although few patients were available for the study, we conclude that therapy with rHuIFN-gamma for 6 months did not enhance the synthesis of NO by NEU and MON in CGD patients. Whether the increased excretion of NO2- and NO3- in the urine of CGD patients after rHUIFN-gamma therapy reflects an induction of NO-synthase in cells other than leukocytes remains to be investigated.|
|Editor:||Mary Ann Liebert Inc Publ|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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