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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleEffect of endometriosis on the protein expression pattern of follicular fluid from patients submitted to controlled ovarian hyperstimulation for in vitro fertilizationpt_BR
dc.contributor.authorLo Turco, EGpt_BR
dc.contributor.authorSouza, GHMFpt_BR
dc.contributor.authorGarcia, JSpt_BR
dc.contributor.authorFerreira, CRpt_BR
dc.contributor.authorEberlin, MNpt_BR
dc.contributor.authorBertolla, RPpt_BR
unicamp.authorLo Turco, Edson Guimaraes Bertolla, Ricardo Pimenta Univ Fed Sao Paulo, Human Reprod Sect, Div Urol, Dept Surg, BR-04039060 Sao Paulo, Brazilpt_BR
unicamp.authorMartins Ferreira Souza, Gustavo Henrique Waters Corp, Mass Spectrometry Applicat Res & Dev Lab, BR-06455020 Sao Paulo, Brazilpt_BR
unicamp.authorMartins Ferreira Souza, Gustavo Henrique Garcia, Jerusa Simone Ferreira, Christina Ramires Eberlin, Marcos Nogueira Univ Estadual Campinas, UNICAMP, Inst Chem, ThoMSon Mass Spectrometry Lab, BR-13083970 Campinas, SP, Brazilpt_BR
unicamp.authorGarcia, Jerusa Simone Alfenas Fed Univ, Dept Exact Sci, BR-37170000 Alfenas, MG, Brazilpt_BR
dc.subjectfollicular fluidpt_BR
dc.subject.wosMature Human Folliclespt_BR
dc.subject.wosProteomic Analysispt_BR
dc.subject.wosIdentification Technologypt_BR
dc.subject.wosInterleukin-1 Receptorpt_BR
dc.description.abstractBACKGROUND: The aim of this study was to evaluate protein expression profile and quantify the proteins present in follicular fluid (FF) samples from women with endometriosis and pregnant women without endometriosis. METHODS: A prospective case control study was carried out including women with Stage III or IV endometriosis (Group I) and pregnant women without endometriosis (Group II), both at the maximum age of 35 years. Women were submitted to controlled ovarian stimulation for in vitro fertilization, and FF was collected after ultrasound-guided ovarian aspiration. FF from both ovaries was pooled, and patient samples were pooled according to Group I or II. Pooled protein samples were separated and analyzed by MudPIT (multidimensional protein identification technology followed by Expression(E) and label-free quantification with ProteinLynxGlobalServer 2.4v, Identity(E) and Expression(E) software). RESULTS: A total of 416 proteins or randomic sequence were identified, 62 proteins differentially expressed between Groups I and II. One ( 1.6%) was expressed at a higher level and 36 (58.1%) were uniquely expressed in Group 1, whereas 8 (12.9%) were expressed at a higher level and 17 (27.4%) were uniquely expressed in Group II. Of all these, 15 (24.2%) are related to binding, I (1.6%) to immune response, 8 (12.9%) to cell division, 3 (4.8%) to cellular metabolism, 16 (25.8%) to general function and 19 (30.6%) do not yet present an identified function. CONCLUSIONS: Protein expression profiles of patients with and without endometriosis identified at least 64 proteins differentially expressed, which may be related to the physiopathology of endometriosis. These proteins may additionally be useful in determining potential biomarkers for diagnostics, as well as for therapeutic intervention in women with infertility due to
dc.relation.ispartofHuman Reproductionpt_BR
dc.relation.ispartofabbreviationHum. Reprod.pt_BR
dc.publisherOxford Univ Presspt_BR
dc.identifier.citationHuman Reproduction. Oxford Univ Press, v. 25, n. 7, n. 1755, n. 1766, 2010.pt_BR
dc.sourceWeb of Sciencept_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipDivision of Urology, Human Reproduction Section at the Sao Paulo Federal Universitypt_BR
dc.description.sponsorshipThoMSon Mass Spectrometry Laboratory of the Institute of Chemistry at the University of Campinaspt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsordocumentnumberCNPq [475 108/2009-4]pt
dc.description.sponsordocumentnumberFAPESP [2008/10756-7]pt
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