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Type: Artigo de periódico
Title: Effect of chaotropic agents on reversible unfolding of a soybean (Glycine max) seed acid phosphatase
Author: Cavagis, ADM
Granjeiro, PA
Ferreira, CV
Aoyama, H
Abstract: In this work we examined the effect of urea and guanidinium chloride on the structural stability of a single isoform of soybean seed acid phosphatase, based on the intensity of tryptophan fluorescence as a function of denaturant concentration. The free energy of unfolding, DeltaG(u), was calculated at 25 degreesC as a function of the concentrations of both chaotropic agents; the conformational stability, DeltaG (H2O), was determined to be 2.48 kcal mol(-1). Center of mass, determined from analysis of fluorescence data, was used as a parameter to assess conformational changes. Our results indicate that complete enzyme inactivation occurred before full enzyme unfolding in both cases, and suggest that there are differences between the conformational flexibility of the active-site and that of the macromolecule as a whole. (C) 2004 Elsevier Ltd. All rights reserved.
Subject: glycine max
acid phosphatase
chaotropic agents
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Rights: fechado
Identifier DOI: 10.1016/j.phytochem.2004.02.004
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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