Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/63716
Type: Artigo de periódico
Title: Effect of a trypsin inhibitor from Dimorphandra mollis seeds on the development of Callosobruchus maculatus
Author: Macedo, MLR
Mello, GC
Freire, MDGM
Novello, JC
Marangoni, S
de Matos, DGG
Abstract: Bruchid larvae cause major losses in grain legume crops throughout the world. Some bruchid species, such as the cowpea weevil, are pests that damage stored seeds. Plants synthesize a variety of molecules, including proteinaceous proteinase inhibitors, to defend themselves against attack by insects. In this work, a trypsin inhibitor (DMTI-II) isolated from Dimorphandra mollis seeds was tested for anti-insect activity against Callosobruchus maculatus larvae. The inhibitor produced ca. 67% mortality to this bruchid when incorporated into an artificial diet at a level of 1%. The doses necessary to cause 50% mortality (LD50) and to reduce weight by 50% (ED50) for DMTI-II were ca. 0.50% and 0.60%, respectively. The action of DMTI-II on C. maculatus larvae may involve the inhibition of trypsin-like activity of larval midgut extracts, the absence of digestion by midgut preparations or with a mixture of pepsin and papain, and its association with a chitin column and chitinous structure in the midgut of this insect. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.
Subject: Callosobruchus maculatus
dimorphandramollis
resistance
trypsin inhibitor
Country: França
Editor: Gauthier-villars/editions Elsevier
Rights: fechado
Identifier DOI: 10.1016/S0981-9428(02)01441-9
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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