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Type: Artigo de periódico
Title: Dual gene transfer of fibroblast growth factor-2 and platelet derived growth factor-BB using plasmid deoxyribonucleic acid promotes effective angiogenesis and arteriogenesis in a rodent model of hindlimb ischemia
Author: De Paula, EV
Flores-Nascimento, MC
Arruda, VR
Garcia, RA
Ramos, CD
Guillaumon, AT
Annichino-Bizzacchi, JM
Abstract: The protein infusion of basic fibroblast growth factor-2 (FGF-2) and platelet derived growth factor-BB (PDGF-BB) have been shown to promote the formation of a stable and functional vascular network in small and large animal models of ischemia. Here, we sought to determine whether a similar effect could be obtained using a gene-therapy-based strategy with nonviral vectors. Rats underwent a surgical procedure to create hindlimb ischemia and were injected with a combination of plasmids that expressed FGF-2 and PDGF-BB. Anatomical and functional parameters of the angiogenesis and arteriogenesis response were evaluated after 4 weeks. The results were compared with rats injected with plasmids that expressed a reporter gene or the extensively studied vascular endothelial growth factor (VEGF165) alone. Treatment with the FGF-2/PDGF-BB combination increased the angiogenesis and arteriogenesis response compared with the empty plasmid, and it was as effective as VEGF165. In terms of safety, the combination allowed the use of a 50% lower individual dose of each plasmid and in addition promoted the formation of more stable vessels than VEGF165. In conclusion, the dual gene transfer of FGF-2 and PDGF-BB using nonviral vectors is safe and effective in promoting the formation of a functional vascular network in a rodent model of hindlimb ischemia. (Translational Research 2009; 153:232-239)
Country: EUA
Editor: Elsevier Science Inc
Citation: Translational Research. Elsevier Science Inc, v. 153, n. 5, n. 232, n. 239, 2009.
Rights: fechado
Identifier DOI: 10.1016/j.trsl.2009.02.002
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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