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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titledsRNA-induced gene silencing in Moniliophthora perniciosa, the causal agent of witches' broom disease of cacaopt_BR
dc.contributor.authordos Santos, ACCpt_BR
dc.contributor.authorSena, JALpt_BR
dc.contributor.authorSantos, SCpt_BR
dc.contributor.authorDias, CVpt_BR
dc.contributor.authorPirovani, CPpt_BR
dc.contributor.authorPungartnik, Cpt_BR
dc.contributor.authorValle, RRpt_BR
dc.contributor.authorCascardo, JCMpt_BR
dc.contributor.authorVincentz, Mpt_BR
unicamp.author.emailcascardo@labbi.uesc.brpt_BR
unicamp.authorCascardo, J. C. M. Univ Estadual Santa Cruz, Dept Ciencias Biol, Lab Genom & Prote, Ctr Biotecnol & Genet, BR-45662000 Ilheus, BA, Brazilpt_BR
unicamp.authorValle, R. R. CEPLAC, Ctr Pesquisas Cacau, BR-45662000 Ilheus, BA, Brazilpt_BR
unicamp.authorVincentz, M. Univ Estadual Campinas, Dept Biol Vegetal, Sao Paulo, Brazilpt_BR
dc.subjectCrinipellis perniciosapt_BR
dc.subjectTheobroma cacaopt_BR
dc.subjectRNAipt_BR
dc.subjectPeroxidasept_BR
dc.subjectGFPpt_BR
dc.subjectHydrophobinspt_BR
dc.subject.wosDouble-stranded-rnapt_BR
dc.subject.wosBasidiomycete Crinipellis-perniciosapt_BR
dc.subject.wosGreen-fluorescent Proteinpt_BR
dc.subject.wosNeurospora-crassapt_BR
dc.subject.wosTheobroma-cacaopt_BR
dc.subject.wosDown-regulationpt_BR
dc.subject.wosPhytophthora-infestanspt_BR
dc.subject.wosCaenorhabditis-eleganspt_BR
dc.subject.wosCladosporium-fulvumpt_BR
dc.subject.wosMagnaporthe-oryzaept_BR
dc.description.abstractThe genome sequence of the hemibiotrophic fungus Moniliophthora perniciosa revealed genes possibly participating in the RNAi machinery. Therefore, studies were performed in order to investigate the efficiency of gene silencing by dsRNA. We showed that the reporter gfp gene stably introduced into the fungus genome can be silenced by transfection of in vitro synthesized gfpdsRNA. In addition, successful dsRNA-induced silencing of endogenous genes coding for hydrophobins and a peroxiredoxin were also achieved. All genes showed a silencing efficiency ranging from 18% to 98% when compared to controls even 28 d after dsRNA treatment, suggesting systemic silencing. Reduction of GFP fluorescence, peroxidase activity levels and survival responses to H(2)O(2) were consistent with the reduction of GFP and peroxidase mRNA levels, respectively. dsRNA transformation of M. perniciosa is shown here to efficiently promote genetic knockdown and can thus be used to assess gene function in this pathogen. (C) 2009 Elsevier Inc. All rights reserved.pt
dc.relation.ispartofFungal Genetics And Biologypt_BR
dc.relation.ispartofabbreviationFungal Genet. Biol.pt_BR
dc.publisher.citySan Diegopt_BR
dc.publisher.countryEUApt_BR
dc.publisherAcademic Press Inc Elsevier Sciencept_BR
dc.date.issued2009pt_BR
dc.date.monthofcirculationNOVpt_BR
dc.identifier.citationFungal Genetics And Biology. Academic Press Inc Elsevier Science, v. 46, n. 11, n. 825, n. 836, 2009.pt_BR
dc.language.isoenpt_BR
dc.description.volume46pt_BR
dc.description.issuenumber11pt_BR
dc.description.firstpage825pt_BR
dc.description.lastpage836pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policypt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn1087-1845pt_BR
dc.identifier.wosidWOS:000273497200002pt_BR
dc.identifier.doi10.1016/j.fgb.2009.06.012pt_BR
dc.description.sponsorshipFINEPpt_BR
dc.description.sponsorshipFA-PESB Mpt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipUNICAMP Genetics Post-Graduation Programpt_BR
dc.description.sponsorshipGenetics Post-Graduation Program of UESCpt_BR
dc.description.sponsorship1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.date.available2014-11-16T01:47:53Z
dc.date.available2015-11-26T16:18:13Z-
dc.date.accessioned2014-11-16T01:47:53Z
dc.date.accessioned2015-11-26T16:18:13Z-
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dc.description.provenanceMade available in DSpace on 2015-11-26T16:18:13Z (GMT). No. of bitstreams: 2 WOS000273497200002.pdf: 1363678 bytes, checksum: e80346f61ea836a514c93e73119912f1 (MD5) WOS000273497200002.pdf.txt: 62987 bytes, checksum: f457c5d5808157654f98c51363bbcc9d (MD5) Previous issue date: 2009en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/63523pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/63523
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/63523-
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