Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/63498
Type: Artigo de periódico
Title: Drosophila mediopunctata microsatellites I: more than a hundred polymorphic loci available for genetic studies
Author: Laborda, PR
Mori, GM
de Souza, AP
Abstract: We describe the first 134 polymorphic microsatellites loci developed for the fly Drosophila mediopunctata. A genomic DNA library double-enriched for dinucleotides was constructed and approximately 600 unambiguous microsatellites were identified. The DNA library consisted of 60% positive clones, short-sized dinucleotides (mean = 8.7 repeats) and 73% of AC/GT combinations. A total of 177 primer pairs were designed and thirteen strains were used to achieve optimal PCR amplification conditions and allelic variability of the isolated loci. The average number of alleles per locus was 4.6 and means of observed and expected heterozygosities were 0.23 and 0.62, respectively. The discriminating power average was 0.72. These new developed markers will be applied in the study of natural population dynamics and for the construction of a molecular linkage map for the species.
Subject: Drosophila
Molecular markers
Enriched genomic library
Microsatellites
Country: Holanda
Editor: Springer
Rights: fechado
Identifier DOI: 10.1007/s12686-009-9070-3
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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