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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleNADPH oxidase activity and cytochrome b(558) content of human epstein-barr-virus-transformed B lymphocytes correlate with expression of genes encoding components of the oxidase systempt_BR
dc.contributor.authorCondino-Neto, Apt_BR
dc.contributor.authorNewburger, PEpt_BR
unicamp.authorUniv Massachusetts, Sch Med, Dept Pediat, Worcester, MA 01605 USA Univ Massachusetts, Sch Med, Ctr Canc, Worcester, MA 01605 USA State Univ Campinas, Ctr Invest Pediat, Sch Med, BR-13081970 Campinas, SP, Brazilpt_BR
dc.subjectrespiratory burstpt_BR
dc.subjectsuperoxidept_BR
dc.subjectfree radicalspt_BR
dc.subjectinflammationpt_BR
dc.subjectphagocytespt_BR
dc.subject.wosChronic Granulomatous-diseasept_BR
dc.subject.wosSuperoxide-generating-systempt_BR
dc.subject.wosRespiratory Burst Oxidasept_BR
dc.subject.wosCell-linespt_BR
dc.subject.wosInterferon-gammapt_BR
dc.subject.wosHuman-neutrophilspt_BR
dc.subject.wosLight Chainpt_BR
dc.subject.wosChromosomal Locationpt_BR
dc.subject.wosActivationpt_BR
dc.subject.wosReceptorpt_BR
dc.description.abstractWe investigated the NADPH oxidase activity, cytochrome b(558) content, and gene expression of gp91-phox and p47-phox in normal Epstein-Barr-virus (EBV)transformed B lymphocytes, compared to EBV-transformed B lymphocytes from patients with X-linked chronic granulomatous disease (CGD), normal peripheral blood neutrophils or mononuclear cells, and the A301 or C8166 lymphoblastoid cell lines. CGD phenotypes included both "classic" disease with no detectable gp91-phox protein (termed X91 degrees) and "variant" phenotype with reduced but detectable gp91-phox protein (X91(-)). Normal EBV-transformed B lymphocytes show a dose-dependent PMA-induced superoxide release. Culturing these cells with IFN-gamma (100 U/ml) and TNF-alpha (1000 U/ml), alone or in combination for 7 days, caused a modest increase in their NADPH oxidase activity (P > 0.05 in all situations). Normal EBV-transformed B lymphocytes have lower NADPH oxidase activity and cytochrome b(558) content than peripheral blood neutrophils or mononuclear cells (P < 0.05 in all situations). In contrast, they have higher NADPH oxidase- activity and cytochrome b(558) content than X91(-)CGD EBV-transformed B lymphocytes (P < 0.05 in all situations). A301 or C8166 lymphoblastoid cell lines and X91 degrees CGD EBV-transformed B lymphocytes have barely detectable NADPH oxidase activity or cytochrome b(558) content (P < 0.05 in all situations). Gene expression studies also show a modest increase in expression and transcription rates of gp91-phox and p47-phox genes in normal EBV-transformed B cells cultured with IFN-gamma (100 U/ml) and TNF-alpha (1000 U/ml), alone or in combination for 7 days. We conclude that NADPH oxidase activity and cytochrome b(558) content correlate with gp(91)-phox and p47-phox gene expression in EBV-transformed B lymphocytes, (C) 1998 Academic Press.pt
dc.relation.ispartofArchives Of Biochemistry And Biophysicspt_BR
dc.relation.ispartofabbreviationArch. Biochem. Biophys.pt_BR
dc.publisher.citySan Diegopt_BR
dc.publisher.countryEUApt_BR
dc.publisherAcademic Press Incpt_BR
dc.date.issued1998pt_BR
dc.date.monthofcirculationDEC 15pt_BR
dc.identifier.citationArchives Of Biochemistry And Biophysics. Academic Press Inc, v. 360, n. 2, n. 158, n. 164, 1998.pt_BR
dc.language.isoenpt_BR
dc.description.volume360pt_BR
dc.description.issuenumber2pt_BR
dc.description.firstpage158pt_BR
dc.description.lastpage164pt_BR
dc.rightsfechadopt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0003-9861pt_BR
dc.identifier.wosidWOS:000077551300002pt_BR
dc.identifier.doi10.1006/abbi.1998.0958pt_BR
dc.date.available2014-12-02T16:25:56Z
dc.date.available2015-11-26T16:25:48Z-
dc.date.accessioned2014-12-02T16:25:56Z
dc.date.accessioned2015-11-26T16:25:48Z-
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dc.description.provenanceMade available in DSpace on 2015-11-26T16:25:48Z (GMT). No. of bitstreams: 2 WOS000077551300002.pdf: 1182256 bytes, checksum: 4d090defcc70f18ebf56f9a897c45a39 (MD5) WOS000077551300002.pdf.txt: 34620 bytes, checksum: e98bb2d483ad1b9915ae8729ea58a3d1 (MD5) Previous issue date: 1998en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/63356pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/63356
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/63356-
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