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Type: | Artigo de periódico |
Title: | N-Acyl-homoserine Lactones from Enterobacter sakazakii (Cronobacter spp.) and Their Degradation by Bacillus cereus Enzymes |
Author: | Araujo, FDS Esper, LMR Kuaye, AY Sircili, MP Marsaioli, AJ |
Abstract: | A chemical study of acyl-homoserine lactones (acyl-HSLs) produced by Enterobacter sakazakii resulted in the identification of three molecules: (S)-N-heptanoyl-HSL, (S)-N-dodecanoyl-HSL and (S)-N-tetradecanoyl-HSL. Mixed cultures of E. sakazakii and Bacillus cereus depleted E. sakazakii acyl-HSLs, suggesting acyl-HSL degradation by B. cereus hydrolases (hydrolysis of the lactone or amide moiety). The expression of B. cereus acyl-HSL lactonase and acyl-homoserine acylase was confirmed by monitoring the biotransformation of (S)-N-dodecanoyl-HSL into (S)-N-dodecanoyl-homoserine, dodecanoic acid and homoserine in the presence of B. cereus whole cells, using electrospray-mass spectrometry (ESI-MS). |
Subject: | Enterobacter sakazakii (Cronobacter spp.) Bacillus cereus N-acyl-homoserine lactones foodborne diseases enzymes |
Country: | EUA |
Editor: | Amer Chemical Soc |
Citation: | Journal Of Agricultural And Food Chemistry. Amer Chemical Soc, v. 60, n. 2, n. 585, n. 592, 2012. |
Rights: | fechado |
Identifier DOI: | 10.1021/jf203846f |
Date Issue: | 2012 |
Appears in Collections: | Unicamp - Artigos e Outros Documentos |
Files in This Item:
File | Description | Size | Format | |
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WOS000299584600006.pdf | 367.68 kB | Adobe PDF | View/Open |
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