Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/62966
Type: Artigo de periódico
Title: CHEMICAL AND PHOTOCHEMICAL GENERATED CARBON-CENTERED RADICAL INTERMEDIATE AND ITS REACTION WITH DEOXYRIBONUCLEIC-ACID
Author: JUSTO, GZ
LIVOTTO, PR
DURAN, N
Abstract: The possible action of carbon-centered radicals in promoting damage to DNA is explored by generation of the 2-phenylethyl radical. The radical is generated during oxidation of phenelzine (2-phenylethyl hydrazine) by ferricyanide, as well as optically from phenylpropionic acid. Covalent binding of the 2-phenylethyl radical to DNA is suggested by studies with the plasmid pBR 322 DNA. Other sensitive techniques used to study DNA damage were the interaction with formaldehyde at 60 degrees C and the fluorescence of DNA-Tb(III) and DNA-DAPI complexes. Theoretical MNDO calculations indicated a preferential attack at position 8 of the guanine residues. This study shows that the 2-phenylethyl radical is able to induce primary effects on nucleic acid structure, leading to alkylated products, especially at purine rings.
Subject: DNA
ALKYLATION
FREE RADICALS
HYDRAZINE
THEORETICAL CALCULATIONS
CARBON-CENTERED RADICAL
2-PHENYLETHYL HYDRAZINE
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Rights: fechado
Identifier DOI: 10.1016/0891-5849(95)00036-W
Date Issue: 1995
Appears in Collections:Unicamp - Artigos e Outros Documentos

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