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Type: Artigo de periódico
Title: Characterization of the insulinotropic action of a phospholipase A(2) isolated from Crotalus durissus collilineatus rattlesnake venom on rat pancreatic islets
Author: Nogueira, TCA
Ferreira, F
Toyama, MH
Stoppiglia, LF
Marangoni, S
Boschero, AC
Cameiro, EM
Abstract: The ability of PLA(2) and crotapotin, isolated from Crotalus durissus collilinearus rattlesnake venom. to stimulate insulin secretion from isolated rat islets was examined. PLA(2) and crotapotin stimulated insulin secretion at 2.8 mmol/L glucose. whereas at a high glucose concentrations (16.7 mmol/L) only PLA(2) stimulated secretion. Nifedipine (10 mumol/L) did not alter the ability of PLA(2) to increase insulin secretion stimulated by a depolarizing concentration of K+ (30 mmol/L). PLAN did not affect (CO2)-C-14 production but significantly increased the efflux of arachidonic acid from isolated islets. These results indicate that PLA(2)-stimulated secretion is not dependent on an additional influx of Ca2+ through L-type Ca2+-channels but rather is associated with arachidonic acid formation in pancreatic islets. (C) 2004 Elsevier Ltd. All rights reserved.
Subject: arachidonic acid
insulin secretion
pancreatic islets
snake venom
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Citation: Toxicon. Pergamon-elsevier Science Ltd, v. 45, n. 2, n. 243, n. 248, 2005.
Rights: fechado
Identifier DOI: 10.1016/j.toxicon.2004.10.017
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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