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Type: Artigo de periódico
Title: Characterization of polyphenol oxidase in coffee
Author: Mazzafera, P
Robinson, SP
Abstract: Polyphenol oxidase (PPO) was characterized in partially purified extracts of leaves (PPO-L) and fruit endosperm (PPO-E) of coffee (Coffea arabica L.). PPO activity was higher in early developmental stages of both leaves and endosperm of fruits. Wounding or exposure of coffee leaves to methyl jasmonate increased PPO activity 1.5-4-fold. PPO was not latent and was not activated by protease treatment. PPO activity was stimulated 10-15% with sodium dodecyl sulphate (SDS) at 0.35-1.75 mM, but at higher concentrations activities were similar to the control samples, without detergent. Prolonged incubation of extracts with trypsin or proteinase K inhibited PPO activity but pepsin had no effect. Inhibition of PPO with proteinase K was increased in the presence of SDS. PPO activity from both tissues was optimal at pH 6-7 and at an assay temperature of 30 degreesC. Activity was highest with chlorogenic acid as substrate with a K-m of 0.882 mM (PPO-L) and 2.27 mM (PPO-E). Hexadecyl trimethyl-ammonium bromide, polyvinylpyrrolidone 40, cinnamic acid and salicylhydroxamic acid inhibited PPO from both tissues. Both enzymes were inactivated by heat but the activity in endosperm extracts was more heat labile than that from leaves. The apparent M-r, determined by gel filtration was 46 (PPO-L) and 50 kDa(PPO-E). Activity-stained SDS-polyacrylamide gel electrophoresis (PAGE) gels and western blots probed with PPO antibodies suggested the existence of a 67 kDa PPO which is susceptible to proteolytic cleavage that generates a 45 kDa active form. (C) 2000 Elsevier Science Ltd. All-rights reserved.
Subject: Coffea arabica
methyl jasmonate
polyphenol oxidase
catechol oxidase
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Citation: Phytochemistry. Pergamon-elsevier Science Ltd, v. 55, n. 4, n. 285, n. 296, 2000.
Rights: fechado
Identifier DOI: 10.1016/S0031-9422(00)00332-0
Date Issue: 2000
Appears in Collections:Unicamp - Artigos e Outros Documentos

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