Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/62864
Type: Artigo de periódico
Title: Characterization of necrosis and ethylene-inducing proteins (NEP) in the basidiomycete Moniliophthora perniciosa, the causal agent of witches' broom in Theobroma cacao
Author: Garcia, O
Macedo, JAN
Tiburcio, R
Zaparoli, G
Rincones, J
Bittencourt, LMC
Ceita, GO
Micheli, F
Gesteira, A
Mariano, AC
Schiavinato, MA
Medrano, FJ
Meinhardt, LW
Pereira, GAG
Cascardo, JCM
Abstract: The hemibiotrophic basidiomycete Moniliophthora perniciosa causes witches' broom disease of Theobroma cacao. Analysis of the M. perniciosa draft genome led to the identification of three putative genes encoding necrosis and ethylene -inducing proteins (MpNEPs), which are apparently located on the same chromosome. MpNEP1 and 2 have highly similar sequences and are able to induce necrosis and ethylene emission in tobacco and cacao leaves. MpNEP1 is expressed in both biotrophic and saprotrophic mycelia, the protein behaves as an oligomer in solution and is very sensitive to temperature. MpNEP2 is expressed mainly in biotrophic mycelia, is present as a monomer in solution at low concentrations (<40 pm) and is able to recover necrosis activity after boiling. These differences indicate that similar NEPs can have distinct physical characteristics and suggest possible complementary roles during the disease development for both proteins. This is the first report of NEP1-like proteins in a basidiomycete. (C) 2007 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
Subject: basidiomycota
cacao
plant pathology
WBD
Country: Inglaterra
Editor: Elsevier Sci Ltd
Rights: fechado
Identifier DOI: 10.1016/j.mycres.2007.01.017
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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