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Type: Artigo de periódico
Title: Characterization of a new family of proteins that interact with the C-terminal region of the chromatin-remodeling factor CHD-3(1)
Author: Lemos, TA
Passos, DO
Nery, FC
Kobarg, J
Abstract: The two human proteins Ki-1/57 and CGI-55 have highly similar amino acid sequences but their functions are unknown. We analyzed them by yeast two-hybrid screens and found that they interact with the C-terminal region of the human chromatin-remodeling factor CHD-3 (chromo-helicase-DNA-binding domain protein-3). The interaction of CGI-55 and CHD-3 could be confirmed in vitro and in vivo by co-immunoprecipitations from Sf9 insect cells. Mapping showed that CGI-55 interacts with CHD-3 via two regions at its N- and C-terminals. The CGI-55 and Ki-1/57 mRNAs show highest expression in muscle, colon and kidney. A CGI55-GFP fusion protein was localized in the cytoplasm, nucleus and perinuclear regions of HeLa cells. These data suggest the possibility that CGI-55 and Ki-1/57 might be involved in nuclear functions like the remodeling of chromatin. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
Subject: chromatin remodeling
protein-protein interaction
domain mapping
cellular localization
Country: Holanda
Editor: Elsevier Science Bv
Citation: Febs Letters. Elsevier Science Bv, v. 533, n. 41699, n. 14, n. 20, 2003.
Rights: fechado
Identifier DOI: 10.1016/S0014-5793(02)03737-7
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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