Please use this identifier to cite or link to this item:
Type: Artigo de periódico
Title: Characterization of a commercial cellulase for hydrolysis of agroindustrial substrates
Author: Balsan, G
Astolfi, V
Benazzi, T
Meireles, MAA
Maugeri, F
Di Luccio, M
Dal Pra, V
Mossi, AJ
Treichel, H
Mazutti, MA
Abstract: This work is focused on the characterization of a commercial cellulase in terms of optimum pH and temperature, stability to pH and temperature and affinity of this enzyme to several substrates, determining the Michaelis-Menten parameters. Maximum activity of cellulase was obtained for the temperature range from 40 to 50 A degrees C and pH from 5.2 to 5.5. Enzyme activity decreased only 15% after 150 h of reaction at temperatures between 30 and 50 A degrees C. No loss of activity was observed at pH 5.0 and 5.5. The cellulase showed satisfactory results in the hydrolysis of agroindustrial substrates, since similar activity was verified on filter paper and other agroindustrial substrates.
Subject: Cellulase
Agroindustrial substrates
Enzymatic hydrolysis
Country: EUA
Editor: Springer
Citation: Bioprocess And Biosystems Engineering. Springer, v. 35, n. 7, n. 1229, n. 1237, 2012.
Rights: fechado
Identifier DOI: 10.1007/s00449-012-0710-8
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
WOS000307514500020.pdf316.16 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.