Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/62594
Type: Artigo de periódico
Title: Direct determination of copper in urine using a sol-gel optical sensor coupled to a multicommutated flow system
Author: Jeronimo, PCA
Araujo, AN
Montenegro, MCBSM
Pasquini, C
Raimundo, IM
Abstract: In this work, a multicommutated flow system incorporating a sol-gel optical sensor is proposed for direct spectrophotometric determination of Cu(II) in urine. The optical sensor was developed by physical entrapment of 4-(2-pyridylazo)resorcinol (PAR) in sol-gel thin films by means of a base-catalysed process. The immobilised PAR formed a red 2:1 complex with Cu(II) with maximum absorbance at 500 nm. Optical transduction was based on a dual-colour light-emitting diode (LED) (green/red) light source and a photodiode detector. The sensor had optimum response and good selectivity towards Cu(II) at pH 7.0 and its regeneration was accomplished with picolinic acid. Linear response was obtained for Cu(II) concentrations between 5.0 and 80.0 mug L-1, with a detection limit of 3.0 mug L-1 and sampling frequency of 14 samples h(-1). Interference from foreign ions was studied at a 10:1 (w/w) ion:Cu(II) ratio. Results obtained from analysis of urine samples were in very good agreement with those obtained by inductively coupled plasma mass spectrometry (ICP-MS); there was no significant differences at a confidence level of 95%.
Subject: Cu(II) optical sensor
sol-gel
4-(2-pyridylazo)resorcinol
multicommutation
urinary copper
Country: Alemanha
Editor: Springer Heidelberg
Rights: fechado
Identifier DOI: 10.1007/s00216-004-2718-7
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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