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Type: Artigo
Title: Dissimilarity between prostaglandin e(1) and nitric-oxide donors as potentiators of plasma exudation in the rabbit skin in-vivo
Author: Mariani- Pedroso, S.R.
Zatz, R.
Bizeto, l.
Antunes, E.
De Nucci, G.
Abstract: The ability of prostaglandin E(1) (PGE(1)) and nitric oxide (NO) donor compounds such as sodium nitroprusside (SNP), glyceryl trinitrate (GTN), and 3-morpholino-sydnonimine (SIN-1) to modulate the histamine- and bradykinin-induced increase in microvascular permeability have been investigated in rabbit skin, The effect of the NO synthesis inhibitor N-omega-nitro-L-arginine methyl ester (L-NAME) on the plasma exudation induced by histamine and bradykinin was also studied. Local edema formation was evaluated using [I-125]human serum albumin, New Zealand white rabbits received an intravenous injection of [I-125]human albumin followed immediately by the intradermal injection of edematogenic agents into the shaved dorsolateral skin. PGE(1) (0.1 nmol/site) significantly potentiated both histamine- and bradykinin-induced edema. In contrast, SNP (0.4-400 nmol/site), SIN-1 (0.4-400 nmol/site), and GTN (0.4-40 nmol/site) did not affect the edematogenic response induced by either histamine or bradykinin. GTN (0.4-40 nmol/site) also had no effect on the increase in plasma exudation induced by histamine and bradykinin in the presence of PGE(1). L-NAME (50-400 nmol/site), but not its enantiomer D-NAME, dose-dependently reduced the edema formation induced by a combination of either histamine or bradykinin with PGE(1). This inhibition was significantly reversed by SNP (4-400 nmol/site) and by high doses (2.5 mu mol/site) of L-arginine (but not by D-arginine). Our results thus demonstrate that PGE(1), but not nitrovasodilators, can actually potentiate histamine- and bradykinin-induced edema in rabbit skin. This discrepancy cannot be explained by the lack of vasodilator activity of the nitrovasodilators since these were able to reverse the L-NAME-induced inhibition of the edema provoked by histamine. Rather, this difference most likely reflects the ability of PGE(1) to modulate vascular permeability by mechanism(s) other than an increase in arterial flow
Subject: Arginina
Country: Reino Unido
Editor: Elsevier
Citation: Prostaglandins Leukotrienes And Essential Fatty Acids. Churchill Livingstone, v. 52, n. 6, n. 399, n. 402, 1995.
Rights: fechado
Identifier DOI: 10.1016/0952-3278(95)90068-3
Date Issue: 1995
Appears in Collections:FCM - Artigos e Outros Documentos

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