Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/62529
Type: Artigo de periódico
Title: DISRUPTION OF CA(2+) HOMEOSTASIS IN TRYPANOSOMA-CRUZI BY CRYSTAL VIOLET
Author: DOCAMPO, R
GADELHA, FR
MORENO, SNJ
BENAIM, G
HOFFMANN, ME
VERCESI, AE
Abstract: We have demonstrated previously that crystal violet induces a rapid, dose-related collapse of the inner mitochondrial membrane potential of Trypanosoma cruzi epimastigotes. In this work, we show that crystal violet-induced dissipation of the membrane potential was accompanied by an efflux of Ca2+ from the mitochondria. In addition, crystal violet inhibited the ATP-dependent, oligomycin-, and antimycin A-insensitive Ca2+ uptake by digitonin-permeabilized epimastigotes. Crystal violet also induced Ca2+ release from the mitochondria and endoplasmic reticulum of digitonin-permeabilized trypomastigotes. Furthermore, crystal violet inhibited Ca2+ uptake and the (Ca2+-Mg2+)-ATPase of a highly enriched plasma membrane fraction of epimastigotes, thus indicating an inhibition of other calcium transport mechanisms of the cells. Disruption of Ca2+ homeostasis by crystal violet may be a key process leading to trypanosome cell injury by this drug.
Subject: MITOCHONDRIA
PLASMA MEMBRANE CA(2+)-ATPASE
FURA-2
Editor: Soc Protozoologists
Rights: fechado
Identifier DOI: 10.1111/j.1550-7408.1993.tb04921.x
Date Issue: 1993
Appears in Collections:Unicamp - Artigos e Outros Documentos

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